The overall objective of this study is to shed more light on the function of ncRNAs in S.cerevisiae. Characterisation of ncRNA function currently lags behind the rate of ncRNA discovery with very few properly understood examples.The first step will be to 43 complete the creation of the ncRNA deletion collection of ~550 ncRNAs. When completed, the collection will contain each ncRNA deletion made in diploid heterozygote, diploid homozygote and both haploid backgrounds. Given the close proximity to SUT/CUTs, a challenge of this work will be to create only deletions which do not affect the expression of neighbouring genes. When completed, the first step will be to collect phenotypic data for the whole collection when grown in a variety of environmental conditions. Developing high-throughput methodology for this will be essential. From the phenotypic screening, ncRNAs can be selected for being the focus of more thorough investigation. This will include investigating the effect the deletion has on the expression of neighbouring genes and the ability of the ncRNA to function in cis or trans. The ncRNA deletion collection and the accompanying phenotypic data will hopefully serve as the building blocks for further research into the function of ncRNA in S.cerevisiae.