Dynamic culture effect in the interaction between breast implant surfaces and breast-derived fibroblasts

UoM administered thesis: Master of Philosophy

  • Authors:
  • Christian Moya Garcia

Abstract

Capsular contracture is a multifactorial post-surgery affliction caused after the constriction of the scar tissue enveloping the mammary implants. Physico-chemical characteristics of breast implants may enact the formation of this complication. Mammary implant shells with smoother surfaces yield a higher rate of capsular contracture. Surface topographies may help to anchor the breast tissue enclosing the implant which are likely to prevent micromotion of the silicone prosthesis. The aim of this research was to investigate the effect of orbital motion, employed to emulate the breast movement pattern during running and walking, in the fibroblast-silicone interaction because there is not a static environment inside the body. Hence, the physico-chemical features of three commercially available silicone breast implant shells with different topographies were analysed to evaluate the interaction of breast-derived fibroblasts and silicone implants after being mechanically stressed. Physico-chemical characterisation of the breast implant surfaces consisted of analysing the surface roughness, wettability and chemistry. The in-vitro characterisation consisted of evaluating the cell adhesion, proliferation, focal adhesion kinase (FAK) and alpha-smooth muscle actin (alpha-SMA) expression after dynamic (orbital motion) and static cultures. The viscosity and density of the culture media were also assessed to determine the shear stress at the bottom of the well-plate produced by the orbital motion. According to the results of this study, breast-derived fibroblasts tend to be susceptible to the physico-chemical characteristics of the silicone shells by adapting to the topographies of the substrate after being mechanically and non-mechanically stressed. Cell adhesion and proliferation enhanced following the application of motion in vitro. The effect of the orbital motion during the incubation time promoted the expression of the FAK, protein related to mechanosensitivity, and the alpha-SMA, cytoskeleton protein akin to contractile forces. The aforementioned may play a role in the manufacture of novel breast implant surfaces which focus on reducing the incidence of afflictions such as capsular contracture.

Details

Original languageEnglish
Awarding Institution
Supervisors/Advisors
Award date31 Dec 2018