Prof Tim HardinghamBSc, PhD, DSc

Assistance, Emeritus Professor

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Publications

  1. 2019
  2. E-pub ahead of print

    Enhanced chondrogenesis from human embryonic stem cells

    Wang, T., Nimkingratana, P., Smith, C. A., Cheng, A., Hardingham, T. & Kimber, S., 9 Jul 2019, In : Stem Cell Research.

    Research output: Contribution to journalArticle

  3. Published
  4. Published

    Identification of long non-coding RNAs expressed in knee and hip osteoarthritic cartilage

    Ajekigbe, B., Cheung, K., Xu, Y., Skelton, A. J., Panagiotopoulos, A., Soul, J., Hardingham, T. E., Deehan, D. J., Barter, M. J. & Young, D. A., 2019, In : Osteoarthritis and Cartilage.

    Research output: Contribution to journalArticle

  5. 2018
  6. Published

    Stratification of knee osteoarthritis: two major patient subgroups identified by genome wide expression analysis of articular cartilage.

    Soul, J., Dunn, S., Anand, S., Serracino-Inglott, F., Schwartz, J-M., Boot-Handford, R. & Hardingham, T., Mar 2018, In : Annals of the rheumatic diseases. 77, p. 423-430

    Research output: Contribution to journalArticle

  7. 2016
  8. Published

    PhenomeScape: a Cytoscape app to identify differentially regulated sub-networks using known disease associations

    Soul, J., Dunn, S., Hardingham, T., Boot-Handford, R. & Schwartz, J-M., 15 Dec 2016, In : Bioinformatics (Oxford, England). 32, 24, p. 3847-9

    Research output: Contribution to journalArticle

  9. Published

    Gene expression changes in damaged osteoarthritic cartilage identify a signature of non-chondrogenic and mechanical responses

    Dunn, S., Soul, J., Anand, S., Schwartz, J-M., Boot-Handford, R. & Hardingham, T., Aug 2016, In : Osteoarthritis and Cartilage. 24, 8, p. 1431-1440 10 p.

    Research output: Contribution to journalArticle

  10. 2015
  11. Published
  12. 2014
  13. Published

    Cartilage Repair Using Human Embryonic Stem Cell-Derived Chondroprogenitors.

    Cheng, A., Kapacee, Z., Peng, J., Lu, S., Lucas, R. J., Hardingham, T. E. & Kimber, S. J., 1 Oct 2014, In : Stem Cells Translational Medicine. p. 1-8 7 p., original article.

    Research output: Contribution to journalArticle

  14. Published

    Assembly of the respiratory Mucin MUC5B a new model for a gel-forming Mucin

    Ridley, C., Kouvatsos, N., Raynal, B. D., Howard, M., Collins, R. F., Desseyn, J. L., Jowitt, T. A., Baldock, C., Davis, C. W., Hardingham, T. E. & Thornton, D. J., 6 Jun 2014, In : Journal of Biological Chemistry. 289, 23, p. 16409-16420 11 p.

    Research output: Contribution to journalArticle

  15. Published

    Generating Cartilage Repair from Pluripotent Stem Cells

    Cheng, A., Hardingham, T. & Kimber, SJ., 2014, In : Tissue Engineering. Part B. Reviews . 20, 4, p. 257-266 9 p.

    Research output: Contribution to journalArticle

  16. 2010
  17. Published

    Order within disorder: Aggrecan chondroitin sulphate-attachment region provides new structural insights into protein sequences classified as disordered

    Jowitt, T. A., Murdoch, A. D., Baldock, C., Berry, R., Day, J. M. & Hardingham, T. E., Dec 2010, In : Proteins: Structure, Function and Bioinformatics. 78, 16, p. 3317-3327 10 p.

    Research output: Contribution to journalArticle

  18. Published

    The potential of stem cells in the treatment of knee cartilage defects

    Khan, W. S., Johnson, D. S. & Hardingham, T. E., Dec 2010, In : The Knee. 17, 6, p. 369-374 5 p.

    Research output: Contribution to journalArticle

  19. Published

    Directed differentiation of human embryonic stem cells toward chondrocytes

    Oldershaw, R. A., Baxter, M. A., Lowe, E. T., Bates, N., Grady, L. M., Soncin, F., Brison, D. R., Hardingham, T. E. & Kimber, S. J., Nov 2010, In : Nature biotechnology. 28, 11, p. 1187-1194 7 p.

    Research output: Contribution to journalArticle

  20. Published

    Fell-Muir lecture: Cartilage 2010 - The known unknowns

    Hardingham, T. E., Jun 2010, In : International Journal of Experimental Pathology. 91, 3, p. 203-209 6 p.

    Research output: Contribution to journalArticle

  21. Published

    Notch signaling during chondrogenesis of human bone marrow stem cells

    Oldershaw, R. A. & Hardingham, T. E., Feb 2010, In : Bone. 46, 2, p. 286-293 7 p.

    Research output: Contribution to journalArticle

  22. Published

    Gene expression in human chondrocytes in late osteoarthritis is changed in both fibrillated and intact cartilage without evidence of generalised chondrocyte hypertrophy

    Brew, C. J., Clegg, P. D., Boot-Handford, R. P., Andrew, J. G. & Hardingham, T., Jan 2010, In : Annals of the rheumatic diseases. 69, 1, p. 234-240 6 p.

    Research output: Contribution to journalArticle

  23. Published

    A chemically-defined protocol for generating chondrocytes from human embryonic stem cells.

    Oldershaw, RA., Baxter, MA., Lowe, ET., Bates, N., Grady, LM., Soncin, F., Brison, DR., Hardingham, TE. & Kimber, SJ., 2010, In : Nature biotechnology. 28, 11

    Research output: Contribution to journalArticle

  24. Published

    Gene expression in human chondrocytes in late OA is changed in both fibrillated and intact cartilages without evidence of generalised chondrocyte hypertrophy

    Brew, CJ., Clegg, PD., Boot-Handford, R., Andrews, TE. & Hardingham, T., 2010, In : Annals Rheum Dis. 467

    Research output: Contribution to journalArticle

  25. Published

    Hardingham T (2010) Cell and Tissue-based Approaches for Cartilage Repair

    Hardingham, T., 2010, In : ALTERNATIVES TO LABORATORY ANIMALS. 38, p. 35-39

    Research output: Contribution to journalArticle

  26. 2009
  27. Published

    The epitope characterisation and the osteogenic differentiation potential of human fat pad-derived stem cells is maintained with ageing in later life

    Khan, W. S., Adesida, A. B., Tew, S. R., Andrew, J. G. & Hardingham, T. E., Feb 2009, In : Injury. 40, 2, p. 150-157 7 p.

    Research output: Contribution to journalArticle

  28. Published

    The influence of donor and hypoxic conditions on the assembly of cartilage matrix by osteoarthritic human articular chondrocytes on Hyalograft matrices

    Katopodi, T., Tew, S. R., Clegg, P. D. & Hardingham, T. E., Feb 2009, In : Biomaterials. 30, 4, p. 535-540 5 p.

    Research output: Contribution to journalArticle

  29. Published

    Hyperosmolarity regulates SOX9 mRNA posttranscriptionally in human articular chondrocytes

    Tew, S. R., Peffers, M. J., McKay, T. R., Lowe, E. T., Khan, W. S., Hardingham, T. E. & Clegg, P. D., 2009, In : American Journal of Physiology: Cell Physiology. 297, 4, p. C898-C906

    Research output: Contribution to journalArticle

  30. Published

    Notch signaling during chondrogenesis of bone marrow stem cells

    Hardingham, T. & Oldershaw, RA., 2009, In : Bone. 350

    Research output: Contribution to journalArticle

  31. 2008
  32. Published

    Hyaluronan binding to Link module of TSG-6 and to G1 domain of aggrecan is differently regulated by pH

    Boon, C. H., Gribbon, P. M., Day, A. J. & Hardingham, T. E., 21 Nov 2008, In : Journal of Biological Chemistry. 283, 47, p. 32294-32301 7 p.

    Research output: Contribution to journalArticle

  33. Published

    SOX9 transduction increases chondroitin sulfate synthesis in cultured human articular chondrocytes without altering glycosyltransferase and sulfotransferase transcription

    Tew, S. R., Pothacharoen, P., Katopodi, T. & Hardingham, T. E., 1 Sep 2008, In : Biochemical Journal. 414, 2, p. 231-236 5 p.

    Research output: Contribution to journalArticle

  34. Published

    Human infrapatellar fat pad-derived stem cells express the pericyte marker 3G5 and show enhanced chondrogenesis after expansion in fibroblast growth factor-2

    Khan, W. S., Tew, S. R., Adesida, A. B. & Hardingham, T. E., 3 Jul 2008, In : Arthritis Research and Therapy. 10, 4, R74.

    Research output: Contribution to journalArticle

  35. Published

    Cellular methods in cartilage research: Primary human chondrocytes in culture and chondrogenesis in human bone marrow stem cells

    Tew, S. R., Murdoch, A. D., Rauchenberg, R. P. & Hardingham, T. E., May 2008, In : Methods. 45, 1, p. 2-9 7 p.

    Research output: Contribution to journalArticle

  36. Published

    Notch signaling through Jagged-1 is necessary to initiate chondrogenesis in human bone marrow stromal cells but must be switched off to complete chondrogenesis

    Oldershaw, R. A., Tew, S. R., Russell, A. M., Meade, K., Hawkins, R., McKay, T. R., Brennan, K. R. & Hardingham, T. E., Mar 2008, In : Stem Cells. 26, 3, p. 666-674 8 p.

    Research output: Contribution to journalArticle

  37. Published

    Extracellular matrix and pathogenic mechanisms in osteoarthritis

    Hardingham, T., Jan 2008, In : Current Rheumatology Reports. 10, 1, p. 30-36 6 p.

    Research output: Contribution to journalArticle

  38. 2007
  39. Published

    Two related but distinct chondroitin sulfate mimetope octasaccharide sequences recognized by monoclonal antibody WF6

    Pothacharoen, P., Kalayanamitra, K., Deepa, S. S., Fukui, S., Hattori, T., Fukushima, N., Hardingham, T., Kongtawelert, P. & Sugahara, K., 30 Nov 2007, In : Journal of Biological Chemistry. 282, 48, p. 35232-35246 14 p.

    Research output: Contribution to journalArticle

  40. Published

    Chondrogenic differentiation of human bone marrow stem cells in transwell cultures: Generation of scaffold-free cartilage

    Murdoch, A. D., Grady, L. M., Ablett, M. P., Katopodi, T., Meadows, R. S. & Hardingham, T. E., Nov 2007, In : Stem Cells. 25, 11, p. 2786-2796 10 p.

    Research output: Contribution to journalArticle

  41. Published

    SOX9 transduction of a human chondrocytic cell line identifies novel genes regulated in primary human chondrocytes and in osteoarthritis

    Tew, S. R., Clegg, P. D., Brew, C. J., Redmond, C. M. & Hardingham, T. E., 12 Oct 2007, In : Arthritis Research and Therapy. 9, 5, R107.

    Research output: Contribution to journalArticle

  42. Published

    Differential cartilaginous tissue formation by human synovial membrane, fat pad, meniscus cells and articular chondrocytes

    Marsano, A., Millward-Sadler, S. J., Salter, D. M., Adesida, A., Hardingham, T., Tognana, E., Kon, E., Chiari-Grisar, C., Nehrer, S., Jakob, M. & Martin, I., Jan 2007, In : Osteoarthritis and Cartilage. 15, 1, p. 48-58 10 p.

    Research output: Contribution to journalArticle

  43. Published

    Human meniscus cells express hypoxia inducible factor-1alpha and increased. SOX9 in response to low oxygen tension in cell aggregate culture

    Adesida, AB., Grady, LM., Khan, WS., Millward-Sadler, SJ., Salter, DM. & Hardingham, TE., 2007, In : Arthritis Research and Therapy. 9, 4

    Research output: Contribution to journalArticle

  44. Published

    Hypoxic conditions increase hypoxia-inducible transcription factor 2alpha and enhance chondrogenesis in stem cells from the infrapatellar fat pad of osteoarthritis patients.

    Khan, WS., Adesida, AB. & Hardingham, TE., 2007, In : Arthritis Research and Therapy. 9, 3

    Research output: Contribution to journalArticle

  45. 2006
  46. Published

    Regulation of SOX9 mRNA in human articular chondrocytes involving p38 MAPK activation and mRNA stabilization

    Tew, S. R. & Hardingham, T. E., 22 Dec 2006, In : Journal of Biological Chemistry. 281, 51, p. 39471-39479 8 p.

    Research output: Contribution to journalArticle

  47. Published

    Cartilage, SOX9 and Notch signals in chondrogenesis

    Hardingham, T. E., Oldershaw, R. A. & Tew, S. R., Oct 2006, In : Journal of Anatomy. 209, 4, p. 469-480 11 p.

    Research output: Contribution to journalArticle

  48. Published

    Raised serum chondroitin sulfate epitope level in ovarian epithelial cancer

    Pothacharoen, P., Siriaunkgul, S., Ong-Chai, S., Supabandhu, J., Kumja, P., Wanaphirak, C., Sugahara, K., Hardingham, T. & Kongtawelert, P., Oct 2006, In : Journal of Biochemistry. 140, 4, p. 517-524 7 p.

    Research output: Contribution to journalArticle

  49. Published

    Solution structure and dynamics of cartilage aggrecan

    Papagiannopoulos, A., Waigh, T. A., Hardingham, T. & Heinrich, M., Jul 2006, In : Biomacromolecules. 7, 7, p. 2162-2172 10 p.

    Research output: Contribution to journalArticle

  50. Published

    The matrix-forming phenotype of cultured human meniscus cells is enhanced after culture with fibroblast growth factor 2 and is further stimulated by hypoxia

    Adesida, A. B., Grady, L. M., Khan, W. S. & Hardingham, T. E., 17 Mar 2006, In : Arthritis Research and Therapy. 8, 3, R61.

    Research output: Contribution to journalArticle

  51. Published

    Raised chondroitin sulfate epitopes and hyaluronan in serum from rheumatoid arthritis and osteoarthritis patients

    Pothacharoen, P., Teekachunhatean, S., Louthrenoo, W., Yingsung, W., Ong-Chai, S., Hardingham, T. & Kongtawelert, P., Mar 2006, In : Osteoarthritis and Cartilage. 14, 3, p. 299-301 2 p.

    Research output: Contribution to journalArticle

  52. 2005
  53. Published

    Retroviral transduction with SOX9 enhances re-expression of the chondrocyte phenotype in passaged osteoarthritic human articular chondrocytes

    Tew, S. R., Li, Y., Pothancharoen, P., Tweats, L. M., Hawkins, R. E. & Hardingham, T. E., Jan 2005, In : Osteoarthritis and Cartilage. 13, 1, p. 80-89 9 p.

    Research output: Contribution to journalArticle

  54. Published

    Human meniscus cells in 3D pellet culture under hypoxia are chondrogenic: this is accompanied by upregulation of prolyl 4-hydroxylase type 1.

    Adesida, A., Tweats, L., Millwards-Sadler, J., Salter, D. & Hardingham, TE., 2005, In : International Journal of Experimental Pathology. 86

    Research output: Contribution to journalArticle

  55. Published

    Stimulation of cartilage matrix synthesis by human articular chondrocytes using SOX9 transfection and hypoxic culture conditions.

    Tew, SR., Li, Y., Tweats, LM., Hawkins, RE. & Hardingham, TE., 2005, In : International Journal of Experimental Pathology. 86

    Research output: Contribution to journalArticle

  56. Published

    The putative role of the notch ligand, jagged 1, in the mediation of the early events of human mesenchymal stem cell chondrogenesis.

    Oldershaw, R., Murdoch, A., Brennan, K. & Hardingham, TE., 2005, In : International Journal of Experimental Pathology. 86

    Research output: Contribution to journalArticle

  57. 2004
  58. Published

    Alternative Splicing in the Aggrecan G3 Domain Influences Binding Interactions with Tenascin-C and Other Extracellular Matrix Proteins

    Day, J. M., Olin, A. I., Murdoch, A. D., Canfield, A., Sasaki, T., Timpl, R., Hardingham, T. E. & Aspberg, A., 26 Mar 2004, In : Journal of Biological Chemistry. 279, 13, p. 12511-12518 7 p.

    Research output: Contribution to journalArticle

  59. Published

    Transduction of Passaged Human Articular Chondrocytes with Adenoviral, Retroviral, and Lentiviral Vectors and the Effects of Enhanced Expression of SOX9

    Ying, L., Tew, S. R., Russell, A. M., Gonzalez, K. R., Hardingham, T. E. & Hawkins, R. E., Mar 2004, In : Tissue Engineering. 10, 3-4, p. 575-584 9 p.

    Research output: Contribution to journalArticle

  60. Published

    Investigating the role of heparan sulfate proteoglycans in hereditary multiple exostoses (HME) tumourigenesis.

    Scholefield, ZM., Hassell, JR., West, L., Govindraj, P., Hecht, JT., Rushton, G., Oldershaw, R., Hardingham, T., Merry, CLR. & Gallagher, JT., 2004, In : International Journal of Experimental Pathology. 85, p. A73-A74

    Research output: Contribution to journalArticle

  61. 2003
  62. Published

    Regional Perspectives on Tissue Engineering: View from a Small Island

    Hardingham, T., Dec 2003, In : Tissue Engineering. 9, 6, p. 1063-1064 1 p.

    Research output: Contribution to journalArticle

  63. Published

    Calcium-dependent protein interactions in MUC5B provide reversible cross-links in salivary mucus

    Raynal, B. D. E., Hardingham, T. E., Sheehan, J. K. & Thornton, D. J., 1 Aug 2003, In : Journal of Biological Chemistry. 278, 31, p. 28703-28710 7 p.

    Research output: Contribution to journalArticle

  64. Published

    Differential regulation of cell-surface proteoglycans by chondrocytes during adaptation to cell culture.

    Murdoch, AD., Oldershaw, RA. & Hardingham, TE., 2003, host publication.

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

  65. Published

    Retroviral SOX9 transduction improves re-expression of type II collagen by passaged human articular chondrocytes in 3-dimensional culture

    Tew, SR., Li, Y., Russell, AM., Prince, JM., Hawkins, RE. & Hardingham, TE., 2003, host publication.

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

  66. 2002
  67. Published

    Concentrated solutions of salivary MUC5B mucin do not replicate the gel-forming properties of saliva

    Raynal, B. D. E., Hardingham, T. E., Thornton, D. J. & Sheehan, J. K., 1 Mar 2002, In : Biochemical Journal. 362, 2, p. 289-296 7 p.

    Research output: Contribution to journalArticle

  68. Published

    Tissue engineering: chondrocytes and cartilage.

    Hardingham, T., Tew, S. & Murdoch, A., 2002, In : Arthritis Research. 4 Suppl 3

    Research output: Contribution to journalArticle

  69. Published

    Tumour necrosis factor alpha stimulates nitric oxide production more potently than interleukin-1beta in porcine articular chondrocytes.

    Goodstone, NJ. & Hardingham, TE., 2002, In : Rheumatology (Oxford, England). 41, 8

    Research output: Contribution to journalArticle

  70. 2001
  71. Published

    Chondrocyte phenotype and cell survival are regulated by culture conditions and by specific cytokines through the expression of Sox-9 transcription factor

    Kolettas, E., Muir, H. I., Barrett, J. C. & Hardingham, T. E., 2001, In : Rheumatology. 40, 10, p. 1146-1156 10 p.

    Research output: Contribution to journalArticle

  72. Published

    Novel confocal-FRAP analysis of carbohydrate-protein interactions within the extracellular matrix.

    Gribbon, P., Heng, B. C. & Hardingham, T. E., 2001, In : Methods in Molecular Biology. 171, p. 487-494 7 p.

    Research output: Contribution to journalArticle

  73. 2000
  74. Published

    The analysis of intermolecular interactions in concentrated hyaluronan solutions suggest no evidence for chain-chain association

    Gribbon, P., Heng, B. C. & Hardingham, T. E., 15 Aug 2000, In : Biochemical Journal. 350, 1, p. 329-335 6 p.

    Research output: Contribution to journalArticle

  75. 1999
  76. Published

    The folded protein modules of the C-terminal G3 domain of aggrecan can each facilitate the translocation and secretion of the extended chondroitin sulfate attachment sequence

    Day, J. M., Murdoch, A. D. & Hardingham, T. E., 31 Dec 1999, In : Journal of Biological Chemistry. 274, 53, p. 38107-38111 4 p.

    Research output: Contribution to journalArticle

  77. 1989
  78. Published

    Structural and immunological studies of keratan sulphates from mature bovine articular cartilage

    Thornton, D. J., Morris, H. G., Cockin, G. H., Huckerby, T. N., Nieduszynski, I. A., Carlstedt, I., Hardingham, T. E. & Ratcliffe, A., 15 May 1989, In : The Biochemical Journal. 260, 1, p. 277-282 6 p.

    Research output: Contribution to journalArticle