Evaluation of complex interventions and natural experiments
Trends in occupational exposures and work-related ill-health
Public and patient involvement in primary care research
Competent in several statistical techniques including multilevel regression modeling, population standardization and SMRs, odds ratios, statistical testing and constructing confidence intervals, specificity and sensitivity analysis, survival analysis, power calculations.
Designing, maintaining and cleaning databases, including data security and compliance with the Data Protection Act.
Experienced in the design, implementation and analysis of RCTs, cross-sectional, case-control, longitudinal and cohort studies.
Use of Microsoft Access, Excel, ARC Gis, Stats Direct, SPSS and Stata.
Writing papers grant applications, ethics applications and reports.
Design and analysis of questionnaires, including administering phone or face to face interviews.
Extracted data from medical records (PCTs and NHS).
Undertaken systematic literature searches and reviews.
Organised the routine aspects of RCTs eg Steering Committees, Data monitoring Committees, randomization schedules etc
Regularly used government statistics such as ONS, NHS, HSE, HPA and DEFRA data.
Recruiting and maintaining participation in difficult groups e.g. infertility patients.
Working with clinicians with minimal time to devote to research.
Supervised research nurses and physiotherapists working on research projects.
Monoclonal and polyclonal antibody production and purification including fusion with polyethylene glycol; selection, freezing and thawing of hybridomas; adaptation to serum free medium; using proteins and haptens to raise polyclonal antibodies in animals and large scale production of monoclonal antibodies by cell culture. Culture of other mammalian cell lines.
Protein purification and characterisation techniques including FPLC; gel-filtration, ion-exchange and affinity chromatography; ultrafiltration; ELISA; RIA; ELRA;Western blot; Southern blot, SDS-PAGE; fluorescent and uv/vis spectrophotometry; antibody fragmentation and separation of the fragments.
Protein derivatisation with polyethylene glycol, dyes, biotin, acrylamide by means of redox polymerization of protein primary amines, cyanogen bromide and haptenisation. Assays to assess the extent of derivatisation such as primary amine estimation or various other amino acid analyses.
Radiolabeling of proteins and cells including iodination of proteins and the labeling of cells with iodine, chromium, tritium and carbon, cell impedance counting, fluorescent labeling of cells and proteins, use of the avidin-biotin interaction. Binding assays and construction of Scatchard plots.
DNA extraction and quantification, surface plasmon resonance
Partitioning in two phase aqueous polymer systems as a means of separating proteins and cells on the basis of their surface chemistry, eg. antigenic differences, and the use of a counter-current distribution apparatus.
HPLC analysis of anthracycline derivatives, chlorambucil, and pH-sensitive dyes in order to measure cellular uptake or intracellular pH. HPLC separation of DNA adducts. Dose-dependent and pH-dependent cytotoxicity assays using cultured cells.