Whole-cell microtiter plate screening assay for terminal hydroxylation of fatty acids by P450sCitation formats

  • External authors:
  • Martin J. Weissenborn
  • Sandra Notonier
  • Sarah Luise Lang
  • Konrad B. Otte
  • Susanne Herter
  • Bernhard Hauer

Standard

Whole-cell microtiter plate screening assay for terminal hydroxylation of fatty acids by P450s. / Weissenborn, Martin J.; Notonier, Sandra; Lang, Sarah Luise; Otte, Konrad B.; Herter, Susanne; Turner, Nicholas; Flitsch, Sabine; Hauer, Bernhard.

In: Chemical Communications, Vol. 52, No. 36, 2016, p. 6158-6161.

Research output: Contribution to journalArticle

Harvard

Weissenborn, MJ, Notonier, S, Lang, SL, Otte, KB, Herter, S, Turner, N, Flitsch, S & Hauer, B 2016, 'Whole-cell microtiter plate screening assay for terminal hydroxylation of fatty acids by P450s' Chemical Communications, vol. 52, no. 36, pp. 6158-6161. https://doi.org/10.1039/c6cc01749e

APA

Weissenborn, M. J., Notonier, S., Lang, S. L., Otte, K. B., Herter, S., Turner, N., ... Hauer, B. (2016). Whole-cell microtiter plate screening assay for terminal hydroxylation of fatty acids by P450s. Chemical Communications, 52(36), 6158-6161. https://doi.org/10.1039/c6cc01749e

Vancouver

Weissenborn MJ, Notonier S, Lang SL, Otte KB, Herter S, Turner N et al. Whole-cell microtiter plate screening assay for terminal hydroxylation of fatty acids by P450s. Chemical Communications. 2016;52(36):6158-6161. https://doi.org/10.1039/c6cc01749e

Author

Weissenborn, Martin J. ; Notonier, Sandra ; Lang, Sarah Luise ; Otte, Konrad B. ; Herter, Susanne ; Turner, Nicholas ; Flitsch, Sabine ; Hauer, Bernhard. / Whole-cell microtiter plate screening assay for terminal hydroxylation of fatty acids by P450s. In: Chemical Communications. 2016 ; Vol. 52, No. 36. pp. 6158-6161.

Bibtex

@article{6c5f5f493827491488dc8960a00a5d55,
title = "Whole-cell microtiter plate screening assay for terminal hydroxylation of fatty acids by P450s",
abstract = "A readily available galactose oxidase (GOase) variant was used to develop a whole cell screening assay. This endpoint detection system was applied in a proof-of-concept approach by screening a focussed mutant library. This led to the discovery of the thus far most active P450 Marinobacter aquaeolei mutant catalysing the terminal hydroxylation of fatty acids.",
author = "Weissenborn, {Martin J.} and Sandra Notonier and Lang, {Sarah Luise} and Otte, {Konrad B.} and Susanne Herter and Nicholas Turner and Sabine Flitsch and Bernhard Hauer",
year = "2016",
doi = "10.1039/c6cc01749e",
language = "English",
volume = "52",
pages = "6158--6161",
journal = "Chemical Communications",
issn = "1359-7345",
publisher = "Royal Society of Chemistry",
number = "36",

}

RIS

TY - JOUR

T1 - Whole-cell microtiter plate screening assay for terminal hydroxylation of fatty acids by P450s

AU - Weissenborn, Martin J.

AU - Notonier, Sandra

AU - Lang, Sarah Luise

AU - Otte, Konrad B.

AU - Herter, Susanne

AU - Turner, Nicholas

AU - Flitsch, Sabine

AU - Hauer, Bernhard

PY - 2016

Y1 - 2016

N2 - A readily available galactose oxidase (GOase) variant was used to develop a whole cell screening assay. This endpoint detection system was applied in a proof-of-concept approach by screening a focussed mutant library. This led to the discovery of the thus far most active P450 Marinobacter aquaeolei mutant catalysing the terminal hydroxylation of fatty acids.

AB - A readily available galactose oxidase (GOase) variant was used to develop a whole cell screening assay. This endpoint detection system was applied in a proof-of-concept approach by screening a focussed mutant library. This led to the discovery of the thus far most active P450 Marinobacter aquaeolei mutant catalysing the terminal hydroxylation of fatty acids.

UR - http://www.scopus.com/inward/record.url?scp=84966318439&partnerID=8YFLogxK

U2 - 10.1039/c6cc01749e

DO - 10.1039/c6cc01749e

M3 - Article

VL - 52

SP - 6158

EP - 6161

JO - Chemical Communications

JF - Chemical Communications

SN - 1359-7345

IS - 36

ER -