Whole-cell microtiter plate screening assay for terminal hydroxylation of fatty acids by P450s

Research output: Contribution to journalArticle

  • External authors:
  • Martin J. Weissenborn
  • Sandra Notonier
  • Sarah Luise Lang
  • Konrad B. Otte
  • Susanne Herter
  • Bernhard Hauer

Abstract

A readily available galactose oxidase (GOase) variant was used to develop a whole cell screening assay. This endpoint detection system was applied in a proof-of-concept approach by screening a focussed mutant library. This led to the discovery of the thus far most active P450 Marinobacter aquaeolei mutant catalysing the terminal hydroxylation of fatty acids.

Bibliographical metadata

Original languageEnglish
Pages (from-to)6158-6161
Number of pages4
JournalChemical Communications
Volume52
Issue number36
Early online date7 Apr 2016
DOIs
StatePublished - 2016