Whole-cell biocatalysts for stereoselective C-H amination reactionsCitation formats

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Whole-cell biocatalysts for stereoselective C-H amination reactions. / Both, Peter; Kelly, Paul; Mutti, Francesco; Turner, Nicholas; Flitsch, Sabine.

In: Angewandte Chemie (International Edition), 18.01.2016.

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Both P, Kelly P, Mutti F, Turner N, Flitsch S. Whole-cell biocatalysts for stereoselective C-H amination reactions. Angewandte Chemie (International Edition). 2016 Jan 18. https://doi.org/10.1002/anie.201510028

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Both, Peter ; Kelly, Paul ; Mutti, Francesco ; Turner, Nicholas ; Flitsch, Sabine. / Whole-cell biocatalysts for stereoselective C-H amination reactions. In: Angewandte Chemie (International Edition). 2016.

Bibtex

@article{253cbabd0a5c4a7f962ba89f1d26dad9,
title = "Whole-cell biocatalysts for stereoselective C-H amination reactions",
abstract = "Enantiomerically pure chiral amines are ubiquitouschemical building blocks in bioactive pharmaceutical products andtheir synthesis from simple starting materials is of great interest. Oneof the most attractive strategies is the stereoselective installation of achiral amine through C-H amination, a transformation which ischemically challenging. Herein we report the application of amultienzyme cascade, generated in a single bacterial whole cellsystem, which is able to catalyse stereoselective benzylic aminationswith ee values of 97.5{\%}. The cascade uses four heterologouslyexpressed recombinant enzymes with cofactors provided by the hostcell and isopropyl amine added as the amine donor. The cascadepresents the first example of the successful de novo design of asingle whole-cell biocatalyst for formal stereoselective C-H amination",
author = "Peter Both and Paul Kelly and Francesco Mutti and Nicholas Turner and Sabine Flitsch",
year = "2016",
month = "1",
day = "18",
doi = "10.1002/anie.201510028",
language = "English",
journal = "Angewandte Chemie, International Edition",
issn = "1433-7851",
publisher = "John Wiley & Sons Ltd",

}

RIS

TY - JOUR

T1 - Whole-cell biocatalysts for stereoselective C-H amination reactions

AU - Both, Peter

AU - Kelly, Paul

AU - Mutti, Francesco

AU - Turner, Nicholas

AU - Flitsch, Sabine

PY - 2016/1/18

Y1 - 2016/1/18

N2 - Enantiomerically pure chiral amines are ubiquitouschemical building blocks in bioactive pharmaceutical products andtheir synthesis from simple starting materials is of great interest. Oneof the most attractive strategies is the stereoselective installation of achiral amine through C-H amination, a transformation which ischemically challenging. Herein we report the application of amultienzyme cascade, generated in a single bacterial whole cellsystem, which is able to catalyse stereoselective benzylic aminationswith ee values of 97.5%. The cascade uses four heterologouslyexpressed recombinant enzymes with cofactors provided by the hostcell and isopropyl amine added as the amine donor. The cascadepresents the first example of the successful de novo design of asingle whole-cell biocatalyst for formal stereoselective C-H amination

AB - Enantiomerically pure chiral amines are ubiquitouschemical building blocks in bioactive pharmaceutical products andtheir synthesis from simple starting materials is of great interest. Oneof the most attractive strategies is the stereoselective installation of achiral amine through C-H amination, a transformation which ischemically challenging. Herein we report the application of amultienzyme cascade, generated in a single bacterial whole cellsystem, which is able to catalyse stereoselective benzylic aminationswith ee values of 97.5%. The cascade uses four heterologouslyexpressed recombinant enzymes with cofactors provided by the hostcell and isopropyl amine added as the amine donor. The cascadepresents the first example of the successful de novo design of asingle whole-cell biocatalyst for formal stereoselective C-H amination

U2 - 10.1002/anie.201510028

DO - 10.1002/anie.201510028

M3 - Article

JO - Angewandte Chemie, International Edition

JF - Angewandte Chemie, International Edition

SN - 1433-7851

ER -