Visualizing gene expression during zebrafish pronephros development and regenerationCitation formats

  • External authors:
  • Amanda N. Marra
  • Brooke E. Chambers
  • Joseph M. Chambers
  • Bridgette E. Drummond
  • Basma D. Adeeb
  • Hannah M. Wesselman
  • Elvin E. Morales
  • Nicole Handa
  • Rebecca A. Wingert

Standard

Visualizing gene expression during zebrafish pronephros development and regeneration. / Marra, Amanda N.; Chambers, Brooke E.; Chambers, Joseph M.; Drummond, Bridgette E.; Adeeb, Basma D.; Wesselman, Hannah M.; Morales, Elvin E.; Handa, Nicole; Pettini, Tom; Ronshaugen, Matthew; Wingert, Rebecca A.

Methods in Cell Biology. ed. / Thomas Weimbs. Elsevier BV, 2019. p. 183-215 (Methods in Cell Biology; Vol. 154).

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

Harvard

Marra, AN, Chambers, BE, Chambers, JM, Drummond, BE, Adeeb, BD, Wesselman, HM, Morales, EE, Handa, N, Pettini, T, Ronshaugen, M & Wingert, RA 2019, Visualizing gene expression during zebrafish pronephros development and regeneration. in T Weimbs (ed.), Methods in Cell Biology. Methods in Cell Biology, vol. 154, Elsevier BV, pp. 183-215. https://doi.org/10.1016/bs.mcb.2019.06.003

APA

Marra, A. N., Chambers, B. E., Chambers, J. M., Drummond, B. E., Adeeb, B. D., Wesselman, H. M., Morales, E. E., Handa, N., Pettini, T., Ronshaugen, M., & Wingert, R. A. (2019). Visualizing gene expression during zebrafish pronephros development and regeneration. In T. Weimbs (Ed.), Methods in Cell Biology (pp. 183-215). (Methods in Cell Biology; Vol. 154). Elsevier BV. https://doi.org/10.1016/bs.mcb.2019.06.003

Vancouver

Marra AN, Chambers BE, Chambers JM, Drummond BE, Adeeb BD, Wesselman HM et al. Visualizing gene expression during zebrafish pronephros development and regeneration. In Weimbs T, editor, Methods in Cell Biology. Elsevier BV. 2019. p. 183-215. (Methods in Cell Biology). https://doi.org/10.1016/bs.mcb.2019.06.003

Author

Marra, Amanda N. ; Chambers, Brooke E. ; Chambers, Joseph M. ; Drummond, Bridgette E. ; Adeeb, Basma D. ; Wesselman, Hannah M. ; Morales, Elvin E. ; Handa, Nicole ; Pettini, Tom ; Ronshaugen, Matthew ; Wingert, Rebecca A. / Visualizing gene expression during zebrafish pronephros development and regeneration. Methods in Cell Biology. editor / Thomas Weimbs. Elsevier BV, 2019. pp. 183-215 (Methods in Cell Biology).

Bibtex

@inbook{e83615d9f88049f4931dd625bba5b76a,
title = "Visualizing gene expression during zebrafish pronephros development and regeneration",
abstract = "The vertebrate kidney is comprised of functional units known as nephrons. Defects in nephron development or activity are a common feature of kidney disease. Current medical treatments are unable to ameliorate the dire consequences of nephron deficit or injury. Although there have been tremendous advancements in our understanding of nephron ontogeny and the response to damage, many significant knowledge gaps still remain. The zebrafish embryo kidney, or pronephros, is an ideal model for many renal development and regeneration studies because it is comprised of nephrons that share conserved features with the nephron units that comprise the mammalian metanephric kidney. In this chapter, we provide an overview about the benefits of using the zebrafish pronephros to study the mechanisms underlying nephrogenesis as well as epithelial repair and regeneration. We subsequently detail methods for the spatiotemporal assessment of gene and protein expression in zebrafish embryos that can be used to extend the understanding of nephron development and disease, and thereby create new opportunities to identify therapeutic strategies for regenerative medicine.",
keywords = "Kidney, Nephrogenesis, Nephron, Pronephros, Regeneration, Segment, Zebrafish",
author = "Marra, {Amanda N.} and Chambers, {Brooke E.} and Chambers, {Joseph M.} and Drummond, {Bridgette E.} and Adeeb, {Basma D.} and Wesselman, {Hannah M.} and Morales, {Elvin E.} and Nicole Handa and Tom Pettini and Matthew Ronshaugen and Wingert, {Rebecca A.}",
year = "2019",
doi = "10.1016/bs.mcb.2019.06.003",
language = "English",
isbn = "9780128203354",
series = "Methods in Cell Biology",
publisher = "Elsevier BV",
pages = "183--215",
editor = "Thomas Weimbs",
booktitle = "Methods in Cell Biology",
address = "Netherlands",

}

RIS

TY - CHAP

T1 - Visualizing gene expression during zebrafish pronephros development and regeneration

AU - Marra, Amanda N.

AU - Chambers, Brooke E.

AU - Chambers, Joseph M.

AU - Drummond, Bridgette E.

AU - Adeeb, Basma D.

AU - Wesselman, Hannah M.

AU - Morales, Elvin E.

AU - Handa, Nicole

AU - Pettini, Tom

AU - Ronshaugen, Matthew

AU - Wingert, Rebecca A.

PY - 2019

Y1 - 2019

N2 - The vertebrate kidney is comprised of functional units known as nephrons. Defects in nephron development or activity are a common feature of kidney disease. Current medical treatments are unable to ameliorate the dire consequences of nephron deficit or injury. Although there have been tremendous advancements in our understanding of nephron ontogeny and the response to damage, many significant knowledge gaps still remain. The zebrafish embryo kidney, or pronephros, is an ideal model for many renal development and regeneration studies because it is comprised of nephrons that share conserved features with the nephron units that comprise the mammalian metanephric kidney. In this chapter, we provide an overview about the benefits of using the zebrafish pronephros to study the mechanisms underlying nephrogenesis as well as epithelial repair and regeneration. We subsequently detail methods for the spatiotemporal assessment of gene and protein expression in zebrafish embryos that can be used to extend the understanding of nephron development and disease, and thereby create new opportunities to identify therapeutic strategies for regenerative medicine.

AB - The vertebrate kidney is comprised of functional units known as nephrons. Defects in nephron development or activity are a common feature of kidney disease. Current medical treatments are unable to ameliorate the dire consequences of nephron deficit or injury. Although there have been tremendous advancements in our understanding of nephron ontogeny and the response to damage, many significant knowledge gaps still remain. The zebrafish embryo kidney, or pronephros, is an ideal model for many renal development and regeneration studies because it is comprised of nephrons that share conserved features with the nephron units that comprise the mammalian metanephric kidney. In this chapter, we provide an overview about the benefits of using the zebrafish pronephros to study the mechanisms underlying nephrogenesis as well as epithelial repair and regeneration. We subsequently detail methods for the spatiotemporal assessment of gene and protein expression in zebrafish embryos that can be used to extend the understanding of nephron development and disease, and thereby create new opportunities to identify therapeutic strategies for regenerative medicine.

KW - Kidney

KW - Nephrogenesis

KW - Nephron

KW - Pronephros

KW - Regeneration

KW - Segment

KW - Zebrafish

UR - http://www.scopus.com/inward/record.url?scp=85069696393&partnerID=8YFLogxK

U2 - 10.1016/bs.mcb.2019.06.003

DO - 10.1016/bs.mcb.2019.06.003

M3 - Chapter

C2 - 31493818

AN - SCOPUS:85069696393

SN - 9780128203354

T3 - Methods in Cell Biology

SP - 183

EP - 215

BT - Methods in Cell Biology

A2 - Weimbs, Thomas

PB - Elsevier BV

ER -