Visualization of global RNA synthesis in a human (mini-) organ in situ by click chemistryCitation formats

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Visualization of global RNA synthesis in a human (mini-) organ in situ by click chemistry. / Purba, Talveen; Marsh, Jack; Ng'andu, Kayumba; Kurinna, Svitlana; Paus, Ralf.

In: BioTechniques, Vol. 65, No. 2, 08.2018, p. 97-100.

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Purba, Talveen ; Marsh, Jack ; Ng'andu, Kayumba ; Kurinna, Svitlana ; Paus, Ralf. / Visualization of global RNA synthesis in a human (mini-) organ in situ by click chemistry. In: BioTechniques. 2018 ; Vol. 65, No. 2. pp. 97-100.

Bibtex

@article{5a5c57161a3441acab76be22d3577f1a,
title = "Visualization of global RNA synthesis in a human (mini-) organ in situ by click chemistry",
abstract = "RNA synthesis can be detected by 5-ethynyl uridine (EU) incorporation and click chemistry. Despite identifying a fundamental functional process, this technique has yet to be widely applied to complex human tissue systems. By incorporating EU into human hair follicle (HF) organs cultured ex vivo, nascent RNA synthesis was detected in situ. EU differentially incorporated across the HF epithelium. Interestingly, RNA synthesis did not correlate with protein synthesis, proliferation or epithelial progenitor cell marker expression. By treating human HFs with the cytotoxic cell cycle inhibitor (R)-CR8, which inhibits transcriptional regulators CDK7 and CDK9, it was further shown that this technique can be used to sensitively detect changes in global RNA synthesis in situ. Together, this work delineates new insights into nascent RNA synthesis within a human (mini)- organ and describes a novel read-out parameter that will enrich future ex vivo human tissue research studies.",
author = "Talveen Purba and Jack Marsh and Kayumba Ng'andu and Svitlana Kurinna and Ralf Paus",
year = "2018",
month = aug,
doi = "10.2144/btn-2018-0025",
language = "English",
volume = "65",
pages = "97--100",
journal = "BioTechniques",
issn = "0736-6205",
publisher = "Eaton Publishing Company",
number = "2",

}

RIS

TY - JOUR

T1 - Visualization of global RNA synthesis in a human (mini-) organ in situ by click chemistry

AU - Purba, Talveen

AU - Marsh, Jack

AU - Ng'andu, Kayumba

AU - Kurinna, Svitlana

AU - Paus, Ralf

PY - 2018/8

Y1 - 2018/8

N2 - RNA synthesis can be detected by 5-ethynyl uridine (EU) incorporation and click chemistry. Despite identifying a fundamental functional process, this technique has yet to be widely applied to complex human tissue systems. By incorporating EU into human hair follicle (HF) organs cultured ex vivo, nascent RNA synthesis was detected in situ. EU differentially incorporated across the HF epithelium. Interestingly, RNA synthesis did not correlate with protein synthesis, proliferation or epithelial progenitor cell marker expression. By treating human HFs with the cytotoxic cell cycle inhibitor (R)-CR8, which inhibits transcriptional regulators CDK7 and CDK9, it was further shown that this technique can be used to sensitively detect changes in global RNA synthesis in situ. Together, this work delineates new insights into nascent RNA synthesis within a human (mini)- organ and describes a novel read-out parameter that will enrich future ex vivo human tissue research studies.

AB - RNA synthesis can be detected by 5-ethynyl uridine (EU) incorporation and click chemistry. Despite identifying a fundamental functional process, this technique has yet to be widely applied to complex human tissue systems. By incorporating EU into human hair follicle (HF) organs cultured ex vivo, nascent RNA synthesis was detected in situ. EU differentially incorporated across the HF epithelium. Interestingly, RNA synthesis did not correlate with protein synthesis, proliferation or epithelial progenitor cell marker expression. By treating human HFs with the cytotoxic cell cycle inhibitor (R)-CR8, which inhibits transcriptional regulators CDK7 and CDK9, it was further shown that this technique can be used to sensitively detect changes in global RNA synthesis in situ. Together, this work delineates new insights into nascent RNA synthesis within a human (mini)- organ and describes a novel read-out parameter that will enrich future ex vivo human tissue research studies.

U2 - 10.2144/btn-2018-0025

DO - 10.2144/btn-2018-0025

M3 - Article

C2 - 30091388

VL - 65

SP - 97

EP - 100

JO - BioTechniques

JF - BioTechniques

SN - 0736-6205

IS - 2

ER -