Vinculins interaction with talin is essential for mammary epithelial differentiation.

Research output: Contribution to journalArticlepeer-review

  • External authors:
  • Pengbo Wang
  • Jian Wu
  • Amber Wood
  • Matthew Jones
  • Robert Pedley
  • Weiping Li
  • Robert Ross

Abstract

Vinculin is an essential component of cell adhesion complexes, where it regulates the strength and stability of adhesions. Whilst the role of vinculin in cell motility is well established, it remains unclear how vinculin contributes to other aspects of tissue function. Here we examine the role of vinculin in mammary epithelial cell phenotype. In these cells, correct adhesion to the extracellular matrix is essential for both the formation of polarised secretory acini and for the transcription of tissue-specific milk protein genes. We show that vinculin, through its interaction with talin, controls milk protein gene expression. However, vinculin is not required for the formation of polarised acini. This work reveals new roles for vinculin that are central to cellular differentiation, and for the ability of cells to interpret their extracellular microenvironment.

Bibliographical metadata

Original languageEnglish
Article number18400
JournalScientific Reports
Volume9
Early online date5 Dec 2019
DOIs
Publication statusPublished - 2020