Tumor vascularity: A histological measure of angiogenesis and hypoxiaCitation formats

  • Authors:
  • C. M L West
  • R. A. Cooper
  • J. A. Loncaster
  • D. P. Wilks
  • M. Bromley

Standard

Tumor vascularity: A histological measure of angiogenesis and hypoxia. / West, C. M L; Cooper, R. A.; Loncaster, J. A.; Wilks, D. P.; Bromley, M.

In: Cancer Research, Vol. 61, No. 7, 01.04.2001, p. 2907-2910.

Research output: Contribution to journalArticlepeer-review

Harvard

West, CML, Cooper, RA, Loncaster, JA, Wilks, DP & Bromley, M 2001, 'Tumor vascularity: A histological measure of angiogenesis and hypoxia', Cancer Research, vol. 61, no. 7, pp. 2907-2910.

APA

West, C. M. L., Cooper, R. A., Loncaster, J. A., Wilks, D. P., & Bromley, M. (2001). Tumor vascularity: A histological measure of angiogenesis and hypoxia. Cancer Research, 61(7), 2907-2910.

Vancouver

West CML, Cooper RA, Loncaster JA, Wilks DP, Bromley M. Tumor vascularity: A histological measure of angiogenesis and hypoxia. Cancer Research. 2001 Apr 1;61(7):2907-2910.

Author

West, C. M L ; Cooper, R. A. ; Loncaster, J. A. ; Wilks, D. P. ; Bromley, M. / Tumor vascularity: A histological measure of angiogenesis and hypoxia. In: Cancer Research. 2001 ; Vol. 61, No. 7. pp. 2907-2910.

Bibtex

@article{49cd22c3e62b45d4bea31468960e1f57,
title = "Tumor vascularity: A histological measure of angiogenesis and hypoxia",
abstract = "In this study we sought to clarify the relationship between tumor vascularity, hypoxia, and angiogenesis in human cervix tumors. Two hypotheses were established: first, that measurement of tumor vascularity can provide a histological assessment of both hypoxia and angiogenesis; and second, that expression of angiogenesis-related proteins will provide a surrogate measure of tumor hypoxia. To test the first hypothesis, we studied the prognostic significance of tumor vascularity measured as both intercapillary distance (ICD; thought to reflect tumor oxygenation) and microvessel density (MVD; the hotspot method that provides a histological assessment of tumor angiogenesis). The relationship was also examined of tumor hypoxia, measured using an Eppendorf needle electrode [percentage of values less than 5 mm Hg (HP5)], with ICD and MVD. To test the second hypothesis we examined the relationship between HP5 and the expression of angiogenesis-associated proteins [vascular endothelial growth factor (VEGF) and platelet-derived endothelial cell growth factor (PD-ECGF)]. All of the biological measurements were made on pretreatment tumors. Analysis of data was carried out using log- rank statistics, Cox multivariate analysis, and Spearman's rank correlation. Both ICD and MVD were significant independent prognostic factors for local control. Patients with poorly vascularized tumors (long ICD) had poor local control (P = 0.042). However, patients with poorly vascularized tumors, measured as low MVD, had good local control (P = 0.036). For 107 patients in whom both of the measurements were obtained on the same tumor sections, ICD and MVD provided independent prognostic information in multivariate analysis. There was a significant correlation between tumor hypoxia and ICD (P <0.005) but not MVD (P = 0.41). There was no relationship between hypoxia and the expression of angiogenic factors (VEGF, PD-ECGF). These analyses show that measurement of tumor vascularity can provide different biological information that is dependent on the method used. It is, therefore, important that studies measuring vascularity should include an appropriate definition. There is no relationship between hypoxia and angiogenesis in advanced carcinoma of the cervix and examining the levels of angiogenic proteins may not have a role in assessing hypoxia in cervix cancer.",
keywords = "physiology: Cell Hypoxia, blood supply: Cervix Neoplasms, biosynthesis: Endothelial Growth Factors, Female, Humans, biosynthesis: Lymphokines, Multivariate Analysis, metabolism: Neovascularization, Pathologic, metabolism: Oxygen, Paraffin Embedding, Prospective Studies, Reproducibility of Results, Research Support, Non-U.S. Gov't, Retrospective Studies, biosynthesis: Thymidine Phosphorylase, Vascular Endothelial Growth Factor A, Vascular Endothelial Growth Factors",
author = "West, {C. M L} and Cooper, {R. A.} and Loncaster, {J. A.} and Wilks, {D. P.} and M. Bromley",
year = "2001",
month = apr,
day = "1",
language = "English",
volume = "61",
pages = "2907--2910",
journal = "Cancer Research",
issn = "0008-5472",
publisher = "American Association for Cancer Research",
number = "7",

}

RIS

TY - JOUR

T1 - Tumor vascularity: A histological measure of angiogenesis and hypoxia

AU - West, C. M L

AU - Cooper, R. A.

AU - Loncaster, J. A.

AU - Wilks, D. P.

AU - Bromley, M.

PY - 2001/4/1

Y1 - 2001/4/1

N2 - In this study we sought to clarify the relationship between tumor vascularity, hypoxia, and angiogenesis in human cervix tumors. Two hypotheses were established: first, that measurement of tumor vascularity can provide a histological assessment of both hypoxia and angiogenesis; and second, that expression of angiogenesis-related proteins will provide a surrogate measure of tumor hypoxia. To test the first hypothesis, we studied the prognostic significance of tumor vascularity measured as both intercapillary distance (ICD; thought to reflect tumor oxygenation) and microvessel density (MVD; the hotspot method that provides a histological assessment of tumor angiogenesis). The relationship was also examined of tumor hypoxia, measured using an Eppendorf needle electrode [percentage of values less than 5 mm Hg (HP5)], with ICD and MVD. To test the second hypothesis we examined the relationship between HP5 and the expression of angiogenesis-associated proteins [vascular endothelial growth factor (VEGF) and platelet-derived endothelial cell growth factor (PD-ECGF)]. All of the biological measurements were made on pretreatment tumors. Analysis of data was carried out using log- rank statistics, Cox multivariate analysis, and Spearman's rank correlation. Both ICD and MVD were significant independent prognostic factors for local control. Patients with poorly vascularized tumors (long ICD) had poor local control (P = 0.042). However, patients with poorly vascularized tumors, measured as low MVD, had good local control (P = 0.036). For 107 patients in whom both of the measurements were obtained on the same tumor sections, ICD and MVD provided independent prognostic information in multivariate analysis. There was a significant correlation between tumor hypoxia and ICD (P <0.005) but not MVD (P = 0.41). There was no relationship between hypoxia and the expression of angiogenic factors (VEGF, PD-ECGF). These analyses show that measurement of tumor vascularity can provide different biological information that is dependent on the method used. It is, therefore, important that studies measuring vascularity should include an appropriate definition. There is no relationship between hypoxia and angiogenesis in advanced carcinoma of the cervix and examining the levels of angiogenic proteins may not have a role in assessing hypoxia in cervix cancer.

AB - In this study we sought to clarify the relationship between tumor vascularity, hypoxia, and angiogenesis in human cervix tumors. Two hypotheses were established: first, that measurement of tumor vascularity can provide a histological assessment of both hypoxia and angiogenesis; and second, that expression of angiogenesis-related proteins will provide a surrogate measure of tumor hypoxia. To test the first hypothesis, we studied the prognostic significance of tumor vascularity measured as both intercapillary distance (ICD; thought to reflect tumor oxygenation) and microvessel density (MVD; the hotspot method that provides a histological assessment of tumor angiogenesis). The relationship was also examined of tumor hypoxia, measured using an Eppendorf needle electrode [percentage of values less than 5 mm Hg (HP5)], with ICD and MVD. To test the second hypothesis we examined the relationship between HP5 and the expression of angiogenesis-associated proteins [vascular endothelial growth factor (VEGF) and platelet-derived endothelial cell growth factor (PD-ECGF)]. All of the biological measurements were made on pretreatment tumors. Analysis of data was carried out using log- rank statistics, Cox multivariate analysis, and Spearman's rank correlation. Both ICD and MVD were significant independent prognostic factors for local control. Patients with poorly vascularized tumors (long ICD) had poor local control (P = 0.042). However, patients with poorly vascularized tumors, measured as low MVD, had good local control (P = 0.036). For 107 patients in whom both of the measurements were obtained on the same tumor sections, ICD and MVD provided independent prognostic information in multivariate analysis. There was a significant correlation between tumor hypoxia and ICD (P <0.005) but not MVD (P = 0.41). There was no relationship between hypoxia and the expression of angiogenic factors (VEGF, PD-ECGF). These analyses show that measurement of tumor vascularity can provide different biological information that is dependent on the method used. It is, therefore, important that studies measuring vascularity should include an appropriate definition. There is no relationship between hypoxia and angiogenesis in advanced carcinoma of the cervix and examining the levels of angiogenic proteins may not have a role in assessing hypoxia in cervix cancer.

KW - physiology: Cell Hypoxia

KW - blood supply: Cervix Neoplasms

KW - biosynthesis: Endothelial Growth Factors

KW - Female

KW - Humans

KW - biosynthesis: Lymphokines

KW - Multivariate Analysis

KW - metabolism: Neovascularization, Pathologic

KW - metabolism: Oxygen

KW - Paraffin Embedding

KW - Prospective Studies

KW - Reproducibility of Results

KW - Research Support, Non-U.S. Gov't

KW - Retrospective Studies

KW - biosynthesis: Thymidine Phosphorylase

KW - Vascular Endothelial Growth Factor A

KW - Vascular Endothelial Growth Factors

M3 - Article

VL - 61

SP - 2907

EP - 2910

JO - Cancer Research

JF - Cancer Research

SN - 0008-5472

IS - 7

ER -