Transcription factor EGR1 directs tendon differentiation and promotes tendon repairCitation formats

  • External authors:
  • Marie Justine Guerquin
  • Benjamin Charvet
  • Geoffroy Nourissat
  • Emmanuelle Havis
  • Olivier Ronsin
  • Marie Ange Bonnin
  • Mathilde Ruggiu
  • Isabel Olivera-Martinez
  • Nicolas Robert
  • Yinhui Lu
  • Tristan Baumberger
  • Levon Doursounian
  • Francis Berenbaum
  • Delphine Duprez

Standard

Transcription factor EGR1 directs tendon differentiation and promotes tendon repair. / Guerquin, Marie Justine; Charvet, Benjamin; Nourissat, Geoffroy; Havis, Emmanuelle; Ronsin, Olivier; Bonnin, Marie Ange; Ruggiu, Mathilde; Olivera-Martinez, Isabel; Robert, Nicolas; Lu, Yinhui; Kadler, Karl E.; Baumberger, Tristan; Doursounian, Levon; Berenbaum, Francis; Duprez, Delphine.

In: Journal of Clinical Investigation, Vol. 123, No. 8, 01.08.2013, p. 3564-3576.

Research output: Contribution to journalArticle

Harvard

Guerquin, MJ, Charvet, B, Nourissat, G, Havis, E, Ronsin, O, Bonnin, MA, Ruggiu, M, Olivera-Martinez, I, Robert, N, Lu, Y, Kadler, KE, Baumberger, T, Doursounian, L, Berenbaum, F & Duprez, D 2013, 'Transcription factor EGR1 directs tendon differentiation and promotes tendon repair' Journal of Clinical Investigation, vol. 123, no. 8, pp. 3564-3576. https://doi.org/10.1172/JCI67521

APA

Guerquin, M. J., Charvet, B., Nourissat, G., Havis, E., Ronsin, O., Bonnin, M. A., ... Duprez, D. (2013). Transcription factor EGR1 directs tendon differentiation and promotes tendon repair. Journal of Clinical Investigation, 123(8), 3564-3576. https://doi.org/10.1172/JCI67521

Vancouver

Guerquin MJ, Charvet B, Nourissat G, Havis E, Ronsin O, Bonnin MA et al. Transcription factor EGR1 directs tendon differentiation and promotes tendon repair. Journal of Clinical Investigation. 2013 Aug 1;123(8):3564-3576. https://doi.org/10.1172/JCI67521

Author

Guerquin, Marie Justine ; Charvet, Benjamin ; Nourissat, Geoffroy ; Havis, Emmanuelle ; Ronsin, Olivier ; Bonnin, Marie Ange ; Ruggiu, Mathilde ; Olivera-Martinez, Isabel ; Robert, Nicolas ; Lu, Yinhui ; Kadler, Karl E. ; Baumberger, Tristan ; Doursounian, Levon ; Berenbaum, Francis ; Duprez, Delphine. / Transcription factor EGR1 directs tendon differentiation and promotes tendon repair. In: Journal of Clinical Investigation. 2013 ; Vol. 123, No. 8. pp. 3564-3576.

Bibtex

@article{335610de6a1f479fa8c4293220617b83,
title = "Transcription factor EGR1 directs tendon differentiation and promotes tendon repair",
abstract = "Tendon formation and repair rely on specific combinations of transcription factors, growth factors, and mechanical parameters that regulate the production and spatial organization of type I collagen. Here, we investigated the function of the zinc finger transcription factor EGR1 in tendon formation, healing, and repair using rodent animal models and mesenchymal stem cells (MSCs). Adult tendons of Egr1-/- mice displayed a deficiency in the expression of tendon genes, including Scx, Col1a1, and Col1a2, and were mechanically weaker compared with their WT littermates. EGR1 was recruited to the Col1a1 and Col2a1 promoters in postnatal mouse tendons in vivo. Egr1 was required for the normal gene response following tendon injury in a mouse model of Achilles tendon healing. Forced Egr1 expression programmed MSCs toward the tendon lineage and promoted the formation of in vitro-engineered tendons from MSCs. The application of EGR1-producing MSCs increased the formation of tendon-like tissues in a rat model of Achilles tendon injury. We provide evidence that the ability of EGR1 to promote tendon differentiation is partially mediated by TGF-β2. This study demonstrates EGR1 involvement in adult tendon formation, healing, and repair and identifies Egr1 as a putative target in tendon repair strategies.",
author = "Guerquin, {Marie Justine} and Benjamin Charvet and Geoffroy Nourissat and Emmanuelle Havis and Olivier Ronsin and Bonnin, {Marie Ange} and Mathilde Ruggiu and Isabel Olivera-Martinez and Nicolas Robert and Yinhui Lu and Kadler, {Karl E.} and Tristan Baumberger and Levon Doursounian and Francis Berenbaum and Delphine Duprez",
year = "2013",
month = "8",
day = "1",
doi = "10.1172/JCI67521",
language = "English",
volume = "123",
pages = "3564--3576",
journal = "The Journal of clinical investigation",
issn = "1558-8238",
publisher = "The American Society for Clinical Investigation",
number = "8",

}

RIS

TY - JOUR

T1 - Transcription factor EGR1 directs tendon differentiation and promotes tendon repair

AU - Guerquin, Marie Justine

AU - Charvet, Benjamin

AU - Nourissat, Geoffroy

AU - Havis, Emmanuelle

AU - Ronsin, Olivier

AU - Bonnin, Marie Ange

AU - Ruggiu, Mathilde

AU - Olivera-Martinez, Isabel

AU - Robert, Nicolas

AU - Lu, Yinhui

AU - Kadler, Karl E.

AU - Baumberger, Tristan

AU - Doursounian, Levon

AU - Berenbaum, Francis

AU - Duprez, Delphine

PY - 2013/8/1

Y1 - 2013/8/1

N2 - Tendon formation and repair rely on specific combinations of transcription factors, growth factors, and mechanical parameters that regulate the production and spatial organization of type I collagen. Here, we investigated the function of the zinc finger transcription factor EGR1 in tendon formation, healing, and repair using rodent animal models and mesenchymal stem cells (MSCs). Adult tendons of Egr1-/- mice displayed a deficiency in the expression of tendon genes, including Scx, Col1a1, and Col1a2, and were mechanically weaker compared with their WT littermates. EGR1 was recruited to the Col1a1 and Col2a1 promoters in postnatal mouse tendons in vivo. Egr1 was required for the normal gene response following tendon injury in a mouse model of Achilles tendon healing. Forced Egr1 expression programmed MSCs toward the tendon lineage and promoted the formation of in vitro-engineered tendons from MSCs. The application of EGR1-producing MSCs increased the formation of tendon-like tissues in a rat model of Achilles tendon injury. We provide evidence that the ability of EGR1 to promote tendon differentiation is partially mediated by TGF-β2. This study demonstrates EGR1 involvement in adult tendon formation, healing, and repair and identifies Egr1 as a putative target in tendon repair strategies.

AB - Tendon formation and repair rely on specific combinations of transcription factors, growth factors, and mechanical parameters that regulate the production and spatial organization of type I collagen. Here, we investigated the function of the zinc finger transcription factor EGR1 in tendon formation, healing, and repair using rodent animal models and mesenchymal stem cells (MSCs). Adult tendons of Egr1-/- mice displayed a deficiency in the expression of tendon genes, including Scx, Col1a1, and Col1a2, and were mechanically weaker compared with their WT littermates. EGR1 was recruited to the Col1a1 and Col2a1 promoters in postnatal mouse tendons in vivo. Egr1 was required for the normal gene response following tendon injury in a mouse model of Achilles tendon healing. Forced Egr1 expression programmed MSCs toward the tendon lineage and promoted the formation of in vitro-engineered tendons from MSCs. The application of EGR1-producing MSCs increased the formation of tendon-like tissues in a rat model of Achilles tendon injury. We provide evidence that the ability of EGR1 to promote tendon differentiation is partially mediated by TGF-β2. This study demonstrates EGR1 involvement in adult tendon formation, healing, and repair and identifies Egr1 as a putative target in tendon repair strategies.

U2 - 10.1172/JCI67521

DO - 10.1172/JCI67521

M3 - Article

VL - 123

SP - 3564

EP - 3576

JO - The Journal of clinical investigation

JF - The Journal of clinical investigation

SN - 1558-8238

IS - 8

ER -