TIAM1 antagonizes TAZ/YAP both in the destruction complex in the cytoplasm and in the nucleus to inhibit invasion of intestinal epithelial cells

Research output: Contribution to journalArticle

  • External authors:
  • Zoi Diamantopoulou
  • Gavin White
  • Muhammad Zaki Hidaya Fadlullah Wilmot
  • Marcel Dreger
  • Karen Pickering
  • Joe Maltas
  • Garry Ashton
  • Ruth MacLeod
  • George S. Baillie
  • Graeme I. Murray
  • Owen J. Sansom

Abstract

Aberrant WNT signaling drives colorectal cancer (CRC). Here, we identify TIAM1 as a critical antagonist of CRC progression through inhibiting TAZ and YAP, effectors of WNT signaling. We demonstrate that TIAM1 shuttles between the cytoplasm and nucleus antagonizing TAZ/YAP by distinct mechanisms in the two compartments. In the cytoplasm, TIAM1 localizes to the destruction complex and promotes TAZ degradation by enhancing its interaction with βTrCP. Nuclear TIAM1 suppresses TAZ/YAP interaction with TEADs, inhibiting expression of TAZ/YAP target genes implicated in epithelial-mesenchymal transition, cell migration, and invasion, and consequently suppresses CRC cell migration and invasion. Importantly, high nuclear TIAM1 in clinical specimens associates with increased CRC patient survival. Together, our findings suggest that in CRC TIAM1 suppresses tumor progression by regulating YAP/TAZ activity.

Bibliographical metadata

Original languageEnglish
Article numbere6
Pages (from-to) 621–634
Number of pages14
JournalCancer Cell
Volume31
Issue number5
Early online date13 Apr 2017
DOIs
Publication statusPublished - 8 May 2017

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