Thyrotropin powers human mitochondriaCitation formats

  • External authors:
  • Burkhard Poeggeler
  • Jana Knuever
  • Erzsébet Gáspár
  • Tamás Bíró
  • Matthias Klinger
  • Eniko Bodó
  • Rudolf J. Wiesner
  • Björn E. Wenzel

Standard

Thyrotropin powers human mitochondria. / Poeggeler, Burkhard; Knuever, Jana; Gáspár, Erzsébet; Bíró, Tamás; Klinger, Matthias; Bodó, Eniko; Wiesner, Rudolf J.; Wenzel, Björn E.; Paus, Ralf.

In: Faseb Journal, Vol. 24, No. 5, 05.2010, p. 1525-1531.

Research output: Contribution to journal › Article › peer-review

Harvard

Poeggeler, B, Knuever, J, Gáspár, E, Bíró, T, Klinger, M, Bodó, E, Wiesner, RJ, Wenzel, BE & Paus, R 2010, 'Thyrotropin powers human mitochondria', Faseb Journal, vol. 24, no. 5, pp. 1525-1531. https://doi.org/10.1096/fj.09-147728

APA

Poeggeler, B., Knuever, J., Gáspár, E., Bíró, T., Klinger, M., Bodó, E., Wiesner, R. J., Wenzel, B. E., & Paus, R. (2010). Thyrotropin powers human mitochondria. Faseb Journal, 24(5), 1525-1531. https://doi.org/10.1096/fj.09-147728

Vancouver

Poeggeler B, Knuever J, Gáspár E, Bíró T, Klinger M, Bodó E et al. Thyrotropin powers human mitochondria. Faseb Journal. 2010 May;24(5):1525-1531. https://doi.org/10.1096/fj.09-147728

Author

Poeggeler, Burkhard ; Knuever, Jana ; Gáspár, Erzsébet ; Bíró, Tamás ; Klinger, Matthias ; Bodó, Eniko ; Wiesner, Rudolf J. ; Wenzel, Björn E. ; Paus, Ralf. / Thyrotropin powers human mitochondria. In: Faseb Journal. 2010 ; Vol. 24, No. 5. pp. 1525-1531.

Bibtex

@article{a5d0106db45d4bdcaad191799a2f59ab,
title = "Thyrotropin powers human mitochondria",
abstract = "Here we demonstrate that the neuropeptide hormone thyrotropin (TSH), which controls thyroid hormone production, exerts a major nonclassical function in mitochondrial biology. Based on transcriptional, ultrastructural, immunohistochemical, and biochemical evidence, TSH up-regulates mitochondrial biogenesis and consequently activity in organ-cultured normal human epidermis in situ. Mitochondrial activity was assessed by measuring 2 key components of the respiratory chain. The abundance of mitochondria was assessed employing 2 independent morphological techniques: counting their numbers in human epidermis by high-magnification light microscopy of skin sections immunostained for mitochondria-selective cytochrome-c-oxidase subunit 1 (MTCO1) and transmission electron microscopy (TEM). Treatment with 10 mU/ml of TSH for 6 d strongly up-regulates the number of light-microscopically visualized, MTCO1-demarcated mitochondria. On the ultrastructural level, TEM confirms that TSH indeed stimulates mitochondrial proliferation and biogenesis in the perinuclear region of human skin epidermal keratinocytes. On the transcriptional level, TSH up-regulates MTCO1 mRNA (quantitative RT-PCR) and significantly enhances complex I and IV (cytochrome-c-oxidase) activity. This study pioneers the concept that mitochondrial energy metabolism and biogenesis in a normal, prototypic human epithelial tissue underlies potent neuroendocrine controls and introduces human skin organ culture as a clinically relevant tool for further exploring this novel research frontier in the control of mitochondrial biology. {\textcopyright} FASEB.",
keywords = "Aging, Hypothalamic-pituitary-thyroid axis, Mitochondrial energy metabolism, Oxygen utilization, Skin",
author = "Burkhard Poeggeler and Jana Knuever and Erzs{\'e}bet G{\'a}sp{\'a}r and Tam{\'a}s B{\'i}r{\'o} and Matthias Klinger and Eniko Bod{\'o} and Wiesner, {Rudolf J.} and Wenzel, {Bj{\"o}rn E.} and Ralf Paus",
year = "2010",
month = may,
doi = "10.1096/fj.09-147728",
language = "English",
volume = "24",
pages = "1525--1531",
journal = "The FASEB journal : official publication of the Federation of American Societies for Experimental Biology",
issn = "0892-6638",
publisher = "Federation of American Societies for Experimental Biology",
number = "5",

}

RIS

TY - JOUR

T1 - Thyrotropin powers human mitochondria

AU - Poeggeler, Burkhard

AU - Knuever, Jana

AU - Gáspár, Erzsébet

AU - Bíró, Tamás

AU - Klinger, Matthias

AU - Bodó, Eniko

AU - Wiesner, Rudolf J.

AU - Wenzel, Björn E.

AU - Paus, Ralf

PY - 2010/5

Y1 - 2010/5

N2 - Here we demonstrate that the neuropeptide hormone thyrotropin (TSH), which controls thyroid hormone production, exerts a major nonclassical function in mitochondrial biology. Based on transcriptional, ultrastructural, immunohistochemical, and biochemical evidence, TSH up-regulates mitochondrial biogenesis and consequently activity in organ-cultured normal human epidermis in situ. Mitochondrial activity was assessed by measuring 2 key components of the respiratory chain. The abundance of mitochondria was assessed employing 2 independent morphological techniques: counting their numbers in human epidermis by high-magnification light microscopy of skin sections immunostained for mitochondria-selective cytochrome-c-oxidase subunit 1 (MTCO1) and transmission electron microscopy (TEM). Treatment with 10 mU/ml of TSH for 6 d strongly up-regulates the number of light-microscopically visualized, MTCO1-demarcated mitochondria. On the ultrastructural level, TEM confirms that TSH indeed stimulates mitochondrial proliferation and biogenesis in the perinuclear region of human skin epidermal keratinocytes. On the transcriptional level, TSH up-regulates MTCO1 mRNA (quantitative RT-PCR) and significantly enhances complex I and IV (cytochrome-c-oxidase) activity. This study pioneers the concept that mitochondrial energy metabolism and biogenesis in a normal, prototypic human epithelial tissue underlies potent neuroendocrine controls and introduces human skin organ culture as a clinically relevant tool for further exploring this novel research frontier in the control of mitochondrial biology. © FASEB.

AB - Here we demonstrate that the neuropeptide hormone thyrotropin (TSH), which controls thyroid hormone production, exerts a major nonclassical function in mitochondrial biology. Based on transcriptional, ultrastructural, immunohistochemical, and biochemical evidence, TSH up-regulates mitochondrial biogenesis and consequently activity in organ-cultured normal human epidermis in situ. Mitochondrial activity was assessed by measuring 2 key components of the respiratory chain. The abundance of mitochondria was assessed employing 2 independent morphological techniques: counting their numbers in human epidermis by high-magnification light microscopy of skin sections immunostained for mitochondria-selective cytochrome-c-oxidase subunit 1 (MTCO1) and transmission electron microscopy (TEM). Treatment with 10 mU/ml of TSH for 6 d strongly up-regulates the number of light-microscopically visualized, MTCO1-demarcated mitochondria. On the ultrastructural level, TEM confirms that TSH indeed stimulates mitochondrial proliferation and biogenesis in the perinuclear region of human skin epidermal keratinocytes. On the transcriptional level, TSH up-regulates MTCO1 mRNA (quantitative RT-PCR) and significantly enhances complex I and IV (cytochrome-c-oxidase) activity. This study pioneers the concept that mitochondrial energy metabolism and biogenesis in a normal, prototypic human epithelial tissue underlies potent neuroendocrine controls and introduces human skin organ culture as a clinically relevant tool for further exploring this novel research frontier in the control of mitochondrial biology. © FASEB.

KW - Aging

KW - Hypothalamic-pituitary-thyroid axis

KW - Mitochondrial energy metabolism

KW - Oxygen utilization

KW - Skin

U2 - 10.1096/fj.09-147728

DO - 10.1096/fj.09-147728

M3 - Article

C2 - 20075194

VL - 24

SP - 1525

EP - 1531

JO - The FASEB journal : official publication of the Federation of American Societies for Experimental Biology

JF - The FASEB journal : official publication of the Federation of American Societies for Experimental Biology

SN - 0892-6638

IS - 5

ER -