Three-Dimensional Structure of the Intercalated Disc Reveals Plicate Domain and Gap Junction Remodeling in Heart Failure

Research output: Contribution to journalArticle

  • External authors:
  • Bernard Davenport
  • Jessica Caldwell
  • Tobias Starborg

Abstract

The intercalated disc (ICD) orchestrates electrochemical and mechanical communication between neighboring cardiac myocytes, properties that are perturbed in heart failure (HF). Although structural data from transmission electron microscopy two-dimensional images have provided valuable insights into the domains forming the ICD, there are currently no three-dimensional (3D) reconstructions for an entire ICD in healthy or diseased hearts. Here, we aimed to understand the link between changes in protein expression in an ovine tachypacing-induced HF model and ultrastructural remodeling of the ICD by determining the 3D intercalated disc architecture using serial block face scanning electron microscopy. In the failing myocardium there is no change to the number of ICDs within the left ventricle, but there is an almost doubling of the number of discs with a surface area of

Bibliographical metadata

Original languageEnglish
Pages (from-to)498-507
Number of pages10
JournalBIOPHYSICAL JOURNAL
Volume108
Issue number3
DOIs
Publication statusPublished - 3 Feb 2015

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