Three-dimensional optoacoustic imaging of nailfold capillaries in systemic sclerosis and its potential for disease differentiation using deep learning

Research output: Contribution to journalArticlepeer-review

  • External authors:
  • Suhanyaa Nitkunanantharajah
  • Katja Haedicke
  • Tonia Moore
  • Joanne Manning
  • Dominik Jüstel
  • Vasilis Ntziachristos
  • Andrea Murray

Abstract

The autoimmune disease systemic sclerosis (SSc) causes microvascular changes that can be easily observed cutaneously at the finger nailfold. Optoacoustic imaging (OAI), a combination of optical and ultrasound imaging, specifically raster-scanning optoacoustic mesoscopy (RSOM), offers a non-invasive high-resolution 3D visualization of capillaries allowing for a better view of microvascular changes and an extraction of volumetric measures. In this study, nailfold capillaries of patients with SSc and healthy controls are imaged and compared with each other for the first time using OAI. The nailfolds of 23 patients with SSc and 19 controls were imaged using RSOM. The acquired images were qualitatively compared to images from state-of-the-art imaging tools for SSc, dermoscopy and high magnification capillaroscopy. Furthermore, the vascular volume in the nailfold capillaries, were computed from the RSOM images. The vascular volumes differ significantly between patients with SSc and healthy controls (0.216 ± 0.085 mm3 and 0.337 ± 0.110 mm3; p < 0.0005). In addition, an artificial neural network was trained to automatically differentiate nailfold images from patients with SSc and healthy controls in order to further assess whether OAI is sensitive enough visualize anatomical differences in the capillaries between the two cohorts. Using transfer learning, the trained model classifies images with an area under the ROC curve of 0.897, and a sensitivity of 0.783 and specificity of 0.895. In conclusion, this study demonstrates the capabilities of RSOM as an imaging tool for SSc and establishes it as a modality that facilitates more in-depth studies into the disease mechanisms and progression.

Bibliographical metadata

Original languageEnglish
Article number16444
JournalScientific Reports
Volume10
DOIs
Publication statusPublished - 5 Oct 2020

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