The use of flow cytometry to examine calcium signalling by TRPV1 in mixed cell populations

Research output: Contribution to journalArticle

  • External authors:
  • Bakri M. Assas
  • Wesam H. Abdulaal
  • Majed H. Wakid
  • Haytham A. Zakai

Abstract

Flow cytometric analysis of calcium mobilisation has been in use for many years in the study of specific receptor engagement or isolated cell:cell communication. However, calcium mobilisation/signaling is key to many cell functions including apoptosis, mobility and immune responses. Here we combine multiplex surface staining of whole spleen with Indo-1 AM to visualise calcium mobilisation and examine calcium signaling in a mixed immune cell culture over time. We demonstrate responses to a TRPV1 agonist in distinct cell subtypes without the need for cell separation. Multi parameter staining alongside Indo-1 AM to demonstrate calcium mobilization allows the study of real time calcium signaling in a complex environment

Bibliographical metadata

Original languageEnglish
Pages (from-to)13-19
Number of pages7
JournalAnalytical Biochemistry
Volume527
Early online date31 Mar 2017
DOIs
Publication statusPublished - 15 Jun 2017