The SARS-COV-2 Spike Protein Binds Sialic Acids and Enables Rapid Detection in a Lateral Flow Point of Care Diagnostic Device: ACS Central Science

Research output: Contribution to journalArticlepeer-review

  • External authors:
  • Alexander N. Baker
  • Sarah-Jane Richards
  • Collette S. Guy
  • Thomas R. Congdon
  • Muhammad Hasan
  • Alexander J. Zwetsloot
  • Angelo Gallo
  • Józef R. Lewandowski
  • Phillip J. Stansfeld
  • Anne Straube
  • Marc Walker
  • Simona Chessa
  • Giulia Pergolizzi
  • Simone Dedola
  • Matthew I. Gibson

Abstract

There is an urgent need to understand the behavior of the novel coronavirus (SARS-COV-2), which is the causative agent of COVID-19, and to develop point-of-care diagnostics. Here, a glyconanoparticle platform is used to discover that N-acetyl neuraminic acid has affinity toward the SARS-COV-2 spike glycoprotein, demonstrating its glycan-binding function. Optimization of the particle size and coating enabled detection of the spike glycoprotein in lateral flow and showed selectivity over the SARS-COV-1 spike protein. Using a virus-like particle and a pseudotyped lentivirus model, paper-based lateral flow detection was demonstrated in under 30 min, showing the potential of this system as a low-cost detection platform.

Bibliographical metadata

Original languageEnglish
JournalACS Central Science
DOIs
Publication statusPublished - 22 Sep 2020

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