The mycotoxin patulin, modulates tight junctions in caco-2 cells

Research output: Contribution to journalArticle

  • Authors:
  • John McLaughlin
  • Daniel Lambert
  • Philip J. Padfield
  • Julian P H Burt
  • Catherine A. O'Neill

Abstract

The mycotoxin patulin is a common contaminant of fruit. Here, we demonstrate that patulin reduces the barrier properties of the intestinal cell line, caco-2 by specific effects on tight junction components. Within 5 h of exposure to 100 μM toxin, the transepithelial electrical resistance of caco-2 monolayers was reduced by approximately 95% and the monolayer became more permeable to FITC-labelled dextrans of 4-40 kDa. Immunoblotting revealed occludin proteolysis and a significant reduction in ZO-1 levels. Patulin had no influence on claudin levels but marked changes in their distribution were observed. These data indicate that patulin decreases the barrier properties of caco-2 monolayers by modulation of the tight junction. © 2008 Elsevier Ltd. All rights reserved.

Bibliographical metadata

Original languageEnglish
Pages (from-to)83-89
Number of pages6
JournalToxicology in Vitro
Volume23
Issue number1
DOIs
Publication statusPublished - Feb 2009