The 'melanocyte-keratin' mystery revisited: Neither normal human epidermal nor hair follicle melanocytes express keratin 16 or keratin 6 in situCitation formats

  • Authors:
  • Y. Ramot
  • E. Gáspár
  • A. Dendorfer
  • L. Langbein
  • R. Paus

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The 'melanocyte-keratin' mystery revisited: Neither normal human epidermal nor hair follicle melanocytes express keratin 16 or keratin 6 in situ. / Ramot, Y.; Gáspár, E.; Dendorfer, A.; Langbein, L.; Paus, R.

In: British Journal of Dermatology, Vol. 161, No. 4, 10.2009, p. 933-938.

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Ramot, Y. ; Gáspár, E. ; Dendorfer, A. ; Langbein, L. ; Paus, R. / The 'melanocyte-keratin' mystery revisited: Neither normal human epidermal nor hair follicle melanocytes express keratin 16 or keratin 6 in situ. In: British Journal of Dermatology. 2009 ; Vol. 161, No. 4. pp. 933-938.

Bibtex

@article{5a23a9fd643b4dbaaf63b36d1a98588f,
title = "The 'melanocyte-keratin' mystery revisited: Neither normal human epidermal nor hair follicle melanocytes express keratin 16 or keratin 6 in situ",
abstract = "Background Keratin family proteins are generally accepted as being restricted to epithelial cells. However, several studies have challenged this paradigm by reporting, for example, that melanoma cells can express keratins and that normal human epidermal melanocytes, which derive from the neural crest, express keratin 16 (K16) in situ. Objectives We wished to confirm or refute that K16 and/or its intermediate filament partner, keratin 6 (K6), are expressed in normal human epidermal and/or hair follicle melanocytes in situ. Methods Cryosections of normal human scalp skin were subjected to highly sensitive double immunohistochemistry with specific antibodies against K16 or K6 and against the melanocyte-specific marker NKI/beteb (gp100). Immunoreactivity (IR) was visualized by conventional light microscopy and confocal fluorescence microscopy. Results Despite the use of different, high-sensitivity immunostaining methods, stringent positive and negative controls, and monospecific, well-characterized antikeratin antibodies, we could detect neither K16 nor K6 IR within intraepidermal or intrafollicular pigment cells of normal human scalp skin. Instead, NKI/beteb+ cells were found to be intimately embedded in foci of K16+ and/or K6+ keratinocytes, which might create the illusion of keratin expression by these cells. Conclusions Human epidermal or hair follicle melanocytes do not express K16 and/or K6 while residing in their natural habitat. {\circledC} 2009 British Association of Dermatologists.",
keywords = "Human hair follicle, Keratinocytes, Keratins, Melanocytes",
author = "Y. Ramot and E. G{\'a}sp{\'a}r and A. Dendorfer and L. Langbein and R. Paus",
year = "2009",
month = "10",
doi = "10.1111/j.1365-2133.2009.09294.x",
language = "English",
volume = "161",
pages = "933--938",
journal = "British Journal of Dermatology",
issn = "0007-0963",
publisher = "John Wiley & Sons Ltd",
number = "4",

}

RIS

TY - JOUR

T1 - The 'melanocyte-keratin' mystery revisited: Neither normal human epidermal nor hair follicle melanocytes express keratin 16 or keratin 6 in situ

AU - Ramot, Y.

AU - Gáspár, E.

AU - Dendorfer, A.

AU - Langbein, L.

AU - Paus, R.

PY - 2009/10

Y1 - 2009/10

N2 - Background Keratin family proteins are generally accepted as being restricted to epithelial cells. However, several studies have challenged this paradigm by reporting, for example, that melanoma cells can express keratins and that normal human epidermal melanocytes, which derive from the neural crest, express keratin 16 (K16) in situ. Objectives We wished to confirm or refute that K16 and/or its intermediate filament partner, keratin 6 (K6), are expressed in normal human epidermal and/or hair follicle melanocytes in situ. Methods Cryosections of normal human scalp skin were subjected to highly sensitive double immunohistochemistry with specific antibodies against K16 or K6 and against the melanocyte-specific marker NKI/beteb (gp100). Immunoreactivity (IR) was visualized by conventional light microscopy and confocal fluorescence microscopy. Results Despite the use of different, high-sensitivity immunostaining methods, stringent positive and negative controls, and monospecific, well-characterized antikeratin antibodies, we could detect neither K16 nor K6 IR within intraepidermal or intrafollicular pigment cells of normal human scalp skin. Instead, NKI/beteb+ cells were found to be intimately embedded in foci of K16+ and/or K6+ keratinocytes, which might create the illusion of keratin expression by these cells. Conclusions Human epidermal or hair follicle melanocytes do not express K16 and/or K6 while residing in their natural habitat. © 2009 British Association of Dermatologists.

AB - Background Keratin family proteins are generally accepted as being restricted to epithelial cells. However, several studies have challenged this paradigm by reporting, for example, that melanoma cells can express keratins and that normal human epidermal melanocytes, which derive from the neural crest, express keratin 16 (K16) in situ. Objectives We wished to confirm or refute that K16 and/or its intermediate filament partner, keratin 6 (K6), are expressed in normal human epidermal and/or hair follicle melanocytes in situ. Methods Cryosections of normal human scalp skin were subjected to highly sensitive double immunohistochemistry with specific antibodies against K16 or K6 and against the melanocyte-specific marker NKI/beteb (gp100). Immunoreactivity (IR) was visualized by conventional light microscopy and confocal fluorescence microscopy. Results Despite the use of different, high-sensitivity immunostaining methods, stringent positive and negative controls, and monospecific, well-characterized antikeratin antibodies, we could detect neither K16 nor K6 IR within intraepidermal or intrafollicular pigment cells of normal human scalp skin. Instead, NKI/beteb+ cells were found to be intimately embedded in foci of K16+ and/or K6+ keratinocytes, which might create the illusion of keratin expression by these cells. Conclusions Human epidermal or hair follicle melanocytes do not express K16 and/or K6 while residing in their natural habitat. © 2009 British Association of Dermatologists.

KW - Human hair follicle

KW - Keratinocytes

KW - Keratins

KW - Melanocytes

U2 - 10.1111/j.1365-2133.2009.09294.x

DO - 10.1111/j.1365-2133.2009.09294.x

M3 - Article

VL - 161

SP - 933

EP - 938

JO - British Journal of Dermatology

JF - British Journal of Dermatology

SN - 0007-0963

IS - 4

ER -