The immunohistochemical expression of DNA-PKcs and Ku (p70/p80) in head and neck cancers: relationships with radiosensitivityCitation formats

  • External authors:
  • Thomas Björk-Eriksson
  • Anders Nilsson
  • Bengt Magnusson
  • Marie Svensson
  • Ewa Karlsson
  • Nicholas Slevin
  • Rolf Lewensohn
  • Claes Mercke

Standard

The immunohistochemical expression of DNA-PKcs and Ku (p70/p80) in head and neck cancers: relationships with radiosensitivity. / Björk-Eriksson, Thomas; West, Catharine; Nilsson, Anders; Magnusson, Bengt; Svensson, Marie; Karlsson, Ewa; Slevin, Nicholas; Lewensohn, Rolf; Mercke, Claes.

In: International Journal of Radiation Oncology Biology Physics, Vol. 45, No. 4, 01.11.1999, p. 1005-1010.

Research output: Contribution to journalArticlepeer-review

Harvard

Björk-Eriksson, T, West, C, Nilsson, A, Magnusson, B, Svensson, M, Karlsson, E, Slevin, N, Lewensohn, R & Mercke, C 1999, 'The immunohistochemical expression of DNA-PKcs and Ku (p70/p80) in head and neck cancers: relationships with radiosensitivity', International Journal of Radiation Oncology Biology Physics, vol. 45, no. 4, pp. 1005-1010. https://doi.org/10.1016/S0360-3016(99)00268-0

APA

Björk-Eriksson, T., West, C., Nilsson, A., Magnusson, B., Svensson, M., Karlsson, E., Slevin, N., Lewensohn, R., & Mercke, C. (1999). The immunohistochemical expression of DNA-PKcs and Ku (p70/p80) in head and neck cancers: relationships with radiosensitivity. International Journal of Radiation Oncology Biology Physics, 45(4), 1005-1010. https://doi.org/10.1016/S0360-3016(99)00268-0

Vancouver

Björk-Eriksson T, West C, Nilsson A, Magnusson B, Svensson M, Karlsson E et al. The immunohistochemical expression of DNA-PKcs and Ku (p70/p80) in head and neck cancers: relationships with radiosensitivity. International Journal of Radiation Oncology Biology Physics. 1999 Nov 1;45(4):1005-1010. https://doi.org/10.1016/S0360-3016(99)00268-0

Author

Björk-Eriksson, Thomas ; West, Catharine ; Nilsson, Anders ; Magnusson, Bengt ; Svensson, Marie ; Karlsson, Ewa ; Slevin, Nicholas ; Lewensohn, Rolf ; Mercke, Claes. / The immunohistochemical expression of DNA-PKcs and Ku (p70/p80) in head and neck cancers: relationships with radiosensitivity. In: International Journal of Radiation Oncology Biology Physics. 1999 ; Vol. 45, No. 4. pp. 1005-1010.

Bibtex

@article{e2320e7bbdf349608d15cecc43e4e6b2,
title = "The immunohistochemical expression of DNA-PKcs and Ku (p70/p80) in head and neck cancers: relationships with radiosensitivity",
abstract = "Purpose: The DNA-PK complex is one of the major pathways by which mammalian cells respond to DNA double-strand breaks induced by ionizing radiation. This study evaluated the relationship between the immunohistochemical expression of the individual components of DNA-PK and cellular radiosensitivity in head and neck cancers.Methods and Materials: Biopsies from patients with previously untreated squamous cell carcinomas of the head and neck were assessed for inherent tumor radiosensitivity measured as the surviving fraction at 2 Gy (SF2) using a soft agar clonogenic assay. Paraffin-embedded tumor material from 64 successfully grown specimens was immunohistochemically stained for expression of DNA-PKcs and Ku (p70/p80). The same tumor material was previously analyzed for the immunohistochemical expression of p53.Results: A significant correlation was found between the degree of expression of DNA-PKcs and Ku (p70/p80) (r = 0.55, p <0.001). There were no overall significant differences in the levels of expression of DNA-PKcs and Ku (p70/p80) in tumors from patients of either sex, different sites, histologies, and stages. No relationship was found between SF2 and the expression of either DNA-PKcs (r = 0.22, p = 0.081) or Ku (p70/p80) (r = 0.064, p = 0.62). Comparison with previous immunohistochemical characterization showed no significant correlations between the expression levels of p53 and either DNA-PKcs (r = 0.093, p = 0.46) or Ku (p70/p80) (r = -0.17, p = 0.17).Conclusions: This study suggests that determining the immunohistochemical expression of DNA-PK in head and neck cancers from multiple sites does not have a role as a predictive assay of tumor in vitro radiosensitivity. Copyright (C) 1999 Elsevier Science Inc.",
keywords = "DNA-PK, Head and neck cancers, Ku, Radiosensitivity",
author = "Thomas Bj{\"o}rk-Eriksson and Catharine West and Anders Nilsson and Bengt Magnusson and Marie Svensson and Ewa Karlsson and Nicholas Slevin and Rolf Lewensohn and Claes Mercke",
year = "1999",
month = nov,
day = "1",
doi = "10.1016/S0360-3016(99)00268-0",
language = "English",
volume = "45",
pages = "1005--1010",
journal = "International Journal of Radiation: Oncology - Biology - Physics",
issn = "0360-3016",
publisher = "Elsevier BV",
number = "4",

}

RIS

TY - JOUR

T1 - The immunohistochemical expression of DNA-PKcs and Ku (p70/p80) in head and neck cancers: relationships with radiosensitivity

AU - Björk-Eriksson, Thomas

AU - West, Catharine

AU - Nilsson, Anders

AU - Magnusson, Bengt

AU - Svensson, Marie

AU - Karlsson, Ewa

AU - Slevin, Nicholas

AU - Lewensohn, Rolf

AU - Mercke, Claes

PY - 1999/11/1

Y1 - 1999/11/1

N2 - Purpose: The DNA-PK complex is one of the major pathways by which mammalian cells respond to DNA double-strand breaks induced by ionizing radiation. This study evaluated the relationship between the immunohistochemical expression of the individual components of DNA-PK and cellular radiosensitivity in head and neck cancers.Methods and Materials: Biopsies from patients with previously untreated squamous cell carcinomas of the head and neck were assessed for inherent tumor radiosensitivity measured as the surviving fraction at 2 Gy (SF2) using a soft agar clonogenic assay. Paraffin-embedded tumor material from 64 successfully grown specimens was immunohistochemically stained for expression of DNA-PKcs and Ku (p70/p80). The same tumor material was previously analyzed for the immunohistochemical expression of p53.Results: A significant correlation was found between the degree of expression of DNA-PKcs and Ku (p70/p80) (r = 0.55, p <0.001). There were no overall significant differences in the levels of expression of DNA-PKcs and Ku (p70/p80) in tumors from patients of either sex, different sites, histologies, and stages. No relationship was found between SF2 and the expression of either DNA-PKcs (r = 0.22, p = 0.081) or Ku (p70/p80) (r = 0.064, p = 0.62). Comparison with previous immunohistochemical characterization showed no significant correlations between the expression levels of p53 and either DNA-PKcs (r = 0.093, p = 0.46) or Ku (p70/p80) (r = -0.17, p = 0.17).Conclusions: This study suggests that determining the immunohistochemical expression of DNA-PK in head and neck cancers from multiple sites does not have a role as a predictive assay of tumor in vitro radiosensitivity. Copyright (C) 1999 Elsevier Science Inc.

AB - Purpose: The DNA-PK complex is one of the major pathways by which mammalian cells respond to DNA double-strand breaks induced by ionizing radiation. This study evaluated the relationship between the immunohistochemical expression of the individual components of DNA-PK and cellular radiosensitivity in head and neck cancers.Methods and Materials: Biopsies from patients with previously untreated squamous cell carcinomas of the head and neck were assessed for inherent tumor radiosensitivity measured as the surviving fraction at 2 Gy (SF2) using a soft agar clonogenic assay. Paraffin-embedded tumor material from 64 successfully grown specimens was immunohistochemically stained for expression of DNA-PKcs and Ku (p70/p80). The same tumor material was previously analyzed for the immunohistochemical expression of p53.Results: A significant correlation was found between the degree of expression of DNA-PKcs and Ku (p70/p80) (r = 0.55, p <0.001). There were no overall significant differences in the levels of expression of DNA-PKcs and Ku (p70/p80) in tumors from patients of either sex, different sites, histologies, and stages. No relationship was found between SF2 and the expression of either DNA-PKcs (r = 0.22, p = 0.081) or Ku (p70/p80) (r = 0.064, p = 0.62). Comparison with previous immunohistochemical characterization showed no significant correlations between the expression levels of p53 and either DNA-PKcs (r = 0.093, p = 0.46) or Ku (p70/p80) (r = -0.17, p = 0.17).Conclusions: This study suggests that determining the immunohistochemical expression of DNA-PK in head and neck cancers from multiple sites does not have a role as a predictive assay of tumor in vitro radiosensitivity. Copyright (C) 1999 Elsevier Science Inc.

KW - DNA-PK

KW - Head and neck cancers

KW - Ku

KW - Radiosensitivity

U2 - 10.1016/S0360-3016(99)00268-0

DO - 10.1016/S0360-3016(99)00268-0

M3 - Article

VL - 45

SP - 1005

EP - 1010

JO - International Journal of Radiation: Oncology - Biology - Physics

JF - International Journal of Radiation: Oncology - Biology - Physics

SN - 0360-3016

IS - 4

ER -