The C-Type lectin receptor MGL senses N-acetylgalactosamine on the unique Staphylococcus aureus ST395 wall teichoic acid.Citation formats

  • External authors:
  • Malgorzata E. Mnich
  • Rob van Dalen
  • David Gerlach
  • Astrid Hendriks
  • Andreas Peschel
  • Jos A. G. van Strijp
  • Nina M. van Sorge

Standard

The C-Type lectin receptor MGL senses N-acetylgalactosamine on the unique Staphylococcus aureus ST395 wall teichoic acid. / Mnich, Malgorzata E.; van Dalen, Rob; Gerlach, David; Hendriks, Astrid; Xia, Guoqing; Peschel, Andreas; van Strijp, Jos A. G.; van Sorge, Nina M.

In: Cellular Microbiology, Vol. 21, No. 10, e13072, 20.06.2019.

Research output: Contribution to journalArticlepeer-review

Harvard

Mnich, ME, van Dalen, R, Gerlach, D, Hendriks, A, Xia, G, Peschel, A, van Strijp, JAG & van Sorge, NM 2019, 'The C-Type lectin receptor MGL senses N-acetylgalactosamine on the unique Staphylococcus aureus ST395 wall teichoic acid.', Cellular Microbiology, vol. 21, no. 10, e13072. https://doi.org/10.1111/cmi.13072

APA

Mnich, M. E., van Dalen, R., Gerlach, D., Hendriks, A., Xia, G., Peschel, A., van Strijp, J. A. G., & van Sorge, N. M. (2019). The C-Type lectin receptor MGL senses N-acetylgalactosamine on the unique Staphylococcus aureus ST395 wall teichoic acid. Cellular Microbiology, 21(10), [e13072]. https://doi.org/10.1111/cmi.13072

Vancouver

Mnich ME, van Dalen R, Gerlach D, Hendriks A, Xia G, Peschel A et al. The C-Type lectin receptor MGL senses N-acetylgalactosamine on the unique Staphylococcus aureus ST395 wall teichoic acid. Cellular Microbiology. 2019 Jun 20;21(10). e13072. https://doi.org/10.1111/cmi.13072

Author

Mnich, Malgorzata E. ; van Dalen, Rob ; Gerlach, David ; Hendriks, Astrid ; Xia, Guoqing ; Peschel, Andreas ; van Strijp, Jos A. G. ; van Sorge, Nina M. / The C-Type lectin receptor MGL senses N-acetylgalactosamine on the unique Staphylococcus aureus ST395 wall teichoic acid. In: Cellular Microbiology. 2019 ; Vol. 21, No. 10.

Bibtex

@article{fa6b75bc01344124816848b0c994b404,
title = "The C-Type lectin receptor MGL senses N-acetylgalactosamine on the unique Staphylococcus aureus ST395 wall teichoic acid.",
abstract = "Staphylococcus aureus is a common skin commensal but is also associated with various skin and soft tissue pathologies. Upon invasion, S. aureus is detected by resident innate immune cells through pattern-recognition receptors (PRRs), although a comprehensive understanding of the specific molecular interactions is lacking. Recently, we demonstrated that the PRR langerin (CD207) on epidermal Langerhans cells senses the conserved β-1,4-linked N-acetylglucosamine (GlcNAc) modification on S. aureus wall teichoic acid (WTA), thereby increasing skin inflammation. Interestingly, the S. aureus ST395 lineage as well as certain species of coagulase-negative staphylococci (CoNS) produce a structurally different WTA molecule, consisting of poly-glycerolphosphate with α-O-N-acetylgalactosamine (GalNAc) residues, which are attached by the glycosyltransferase TagN. Here, we demonstrate that S. aureus ST395 strains interact with the human Macrophage Galactose-type lectin (MGL; CD301) receptor, which is expressed by dendritic cells and macrophages in the dermis. MGL bound S. aureus ST395 in a tagN- and GalNAc-dependent manner but did not interact with different tagN-positive CoNS species. However, heterologous expression of S. lugdunensis tagN in S. aureus conferred phage infection and MGL binding, confirming the role of this CoNS enzyme as GalNAc-transferase. Functionally, the detection of GalNAc on S. aureus ST395 WTA by human monocyte-derived dendritic cells significantly enhanced cytokine production. Together, our findings highlight differential recognition of S. aureus glycoprofiles by specific human innate receptors, which may affect downstream adaptive immune responses and pathogen clearance.",
keywords = "teichoic acid, c-type lectin receptor, Macrophage Galactose-type lectin, CD301",
author = "Mnich, {Malgorzata E.} and {van Dalen}, Rob and David Gerlach and Astrid Hendriks and Guoqing Xia and Andreas Peschel and {van Strijp}, {Jos A. G.} and {van Sorge}, {Nina M.}",
year = "2019",
month = jun,
day = "20",
doi = "10.1111/cmi.13072",
language = "English",
volume = "21",
journal = "Cellular Microbiology",
issn = "1462-5822",
publisher = "John Wiley & Sons Ltd",
number = "10",

}

RIS

TY - JOUR

T1 - The C-Type lectin receptor MGL senses N-acetylgalactosamine on the unique Staphylococcus aureus ST395 wall teichoic acid.

AU - Mnich, Malgorzata E.

AU - van Dalen, Rob

AU - Gerlach, David

AU - Hendriks, Astrid

AU - Xia, Guoqing

AU - Peschel, Andreas

AU - van Strijp, Jos A. G.

AU - van Sorge, Nina M.

PY - 2019/6/20

Y1 - 2019/6/20

N2 - Staphylococcus aureus is a common skin commensal but is also associated with various skin and soft tissue pathologies. Upon invasion, S. aureus is detected by resident innate immune cells through pattern-recognition receptors (PRRs), although a comprehensive understanding of the specific molecular interactions is lacking. Recently, we demonstrated that the PRR langerin (CD207) on epidermal Langerhans cells senses the conserved β-1,4-linked N-acetylglucosamine (GlcNAc) modification on S. aureus wall teichoic acid (WTA), thereby increasing skin inflammation. Interestingly, the S. aureus ST395 lineage as well as certain species of coagulase-negative staphylococci (CoNS) produce a structurally different WTA molecule, consisting of poly-glycerolphosphate with α-O-N-acetylgalactosamine (GalNAc) residues, which are attached by the glycosyltransferase TagN. Here, we demonstrate that S. aureus ST395 strains interact with the human Macrophage Galactose-type lectin (MGL; CD301) receptor, which is expressed by dendritic cells and macrophages in the dermis. MGL bound S. aureus ST395 in a tagN- and GalNAc-dependent manner but did not interact with different tagN-positive CoNS species. However, heterologous expression of S. lugdunensis tagN in S. aureus conferred phage infection and MGL binding, confirming the role of this CoNS enzyme as GalNAc-transferase. Functionally, the detection of GalNAc on S. aureus ST395 WTA by human monocyte-derived dendritic cells significantly enhanced cytokine production. Together, our findings highlight differential recognition of S. aureus glycoprofiles by specific human innate receptors, which may affect downstream adaptive immune responses and pathogen clearance.

AB - Staphylococcus aureus is a common skin commensal but is also associated with various skin and soft tissue pathologies. Upon invasion, S. aureus is detected by resident innate immune cells through pattern-recognition receptors (PRRs), although a comprehensive understanding of the specific molecular interactions is lacking. Recently, we demonstrated that the PRR langerin (CD207) on epidermal Langerhans cells senses the conserved β-1,4-linked N-acetylglucosamine (GlcNAc) modification on S. aureus wall teichoic acid (WTA), thereby increasing skin inflammation. Interestingly, the S. aureus ST395 lineage as well as certain species of coagulase-negative staphylococci (CoNS) produce a structurally different WTA molecule, consisting of poly-glycerolphosphate with α-O-N-acetylgalactosamine (GalNAc) residues, which are attached by the glycosyltransferase TagN. Here, we demonstrate that S. aureus ST395 strains interact with the human Macrophage Galactose-type lectin (MGL; CD301) receptor, which is expressed by dendritic cells and macrophages in the dermis. MGL bound S. aureus ST395 in a tagN- and GalNAc-dependent manner but did not interact with different tagN-positive CoNS species. However, heterologous expression of S. lugdunensis tagN in S. aureus conferred phage infection and MGL binding, confirming the role of this CoNS enzyme as GalNAc-transferase. Functionally, the detection of GalNAc on S. aureus ST395 WTA by human monocyte-derived dendritic cells significantly enhanced cytokine production. Together, our findings highlight differential recognition of S. aureus glycoprofiles by specific human innate receptors, which may affect downstream adaptive immune responses and pathogen clearance.

KW - teichoic acid

KW - c-type lectin receptor

KW - Macrophage Galactose-type lectin

KW - CD301

U2 - 10.1111/cmi.13072

DO - 10.1111/cmi.13072

M3 - Article

VL - 21

JO - Cellular Microbiology

JF - Cellular Microbiology

SN - 1462-5822

IS - 10

M1 - e13072

ER -