The continuous oxidation of HMF to FDCA and the immobilisation and stabilisation of periplasmic aldehyde oxidase (PaoABC)

Research output: Contribution to journalArticle

  • External authors:
  • S. M. McKenna
  • P. Mines
  • P. Law
  • K. Kovacs-Schreiner
  • W. R. Birmingham
  • S. Leimkühler
  • A. J. Carnell


By manipulating the reaction conditions, furandicarboxylic acid (FDCA) was prepared by the biooxidation of hydroxymethyl furfural (HMF) in a continuous one-pot reaction using galactose oxidase M3-5, periplasmic aldehyde oxidase (PaoABC), catalase and horseradish peroxidase. In addition, PaoABC was successfully entrapped in a SiO2 hydrogel and recycled 14 times without loss of activity. The catalyst was able to tolerate up to 200 mM DFF concentration giving FDCA in full conversion with very promising TOF and TON values.

Bibliographical metadata

Original languageEnglish
Pages (from-to)4660-4665
Number of pages6
JournalGreen Chemistry
Issue number19
Early online date23 Aug 2017
Publication statusPublished - 2017