The characterisation of a galactokinase from Streptomyces coelicolor

Research output: Contribution to journalArticle

  • External authors:
  • Tessa Keenan
  • Rhys Mills
  • Emily Pocock
  • Darshita Budhadev
  • Martin Fascione

Abstract

Promiscuous galactokinases (GalKs), which catalyse the ATP dependent phosphorylation of galactose in nature, have been widely exploited in biotechnology for the rapid synthesis of diverse sugar-1-phosphates. This work focuses on the characterisation of a bacterial GalK from Streptomyces coelicolor (ScGalK), which was overproduced in Escherichia coli and shown to phosphorylate galactose. ScGalK displayed a broad substrate tolerance, with activity towards Gal, GalN, Gal3D, GalNAc, Man and L-Ara. Most interestingly, ScGalK demonstrated a high activity over a broad pH and temperature range, suggesting that the enzyme could be highly amenable to multi-enzyme systems.

Bibliographical metadata

Original languageEnglish
Pages (from-to)132-137
Number of pages5
JournalCARBOHYDRATE RESEARCH
Volume472
Early online date14 Dec 2018
DOIs
Publication statusPublished - 15 Jan 2019