Syncytial nuclear aggregates in normal placenta show increased nuclear condensation, but apoptosis and cytoskeletal redistribution are uncommonCitation formats

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  • S. J. Coleman
  • L. Gerza

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@article{4a674e8cf7874ff3a9cbe380b3797bbf,
title = "Syncytial nuclear aggregates in normal placenta show increased nuclear condensation, but apoptosis and cytoskeletal redistribution are uncommon",
abstract = "Introduction: Syncytial nuclear aggregates (SNAs) are increased in pregnancy complications; however, little is known about their origin or function. This study aimed to characterise SNAs in more detail than has been reported previously. Methods: Immunohistochemistry and morphological examination at the light and ultrastructural level were used to determine the nature and structure of SNAs. Results: SNAs comprising bridges and syncytial knots had similar frequency with 974 per mm3 of villous tissue (IQR 717-1193) and 833 per mm3 (IQR 766-1190), respectively while there were approximately four times as many sectioning artefacts than knots and bridges combined. SNAs had increased proportions of condensed nuclei compared to the remaining syncytiotrophoblast (33.3{\%} vs. 8.9{\%}) and decreased proportions of euchromatic nuclei (0.0{\%} vs. 16.2{\%}), as assessed by examination of an electron micrograph archive. SNAs showed little evidence of apoptosis, with weak positivity for the apoptosis markers M30-neoepitope at 16.6{\%} and TUNEL at 10.0{\%}; strong staining was rarely seen for either marker. Immunofluorescence demonstrated rare association of actin (α, β or γ) with SNAs, whereas tubulin was in close proximity to SNAs and cytokeratin was seen within and surrounding SNAs. Discussion: M30-positive SNAs traced through serial sections were significantly more likely to be syncytial knots or sectioning artefacts than bridges. Nuclei within SNAs showed signs consistent with degeneration; however, this is unlikely to be an apoptotic process. There are few changes in configuration of cytoskeletal proteins around SNAs. Conclusions: These data suggest that the biogenesis and functional significance of SNAs still require resolution. {\circledC}2013 Elsevier Ltd. All rights reserved.",
keywords = "Apoptosis, Cytoskeletal proteins, Syncytial bridges, Syncytial knots, Syncytiotrophoblast",
author = "Coleman, {S. J.} and L. Gerza and Jones, {C. J P} and Sibley, {C. P.} and Aplin, {J. D.} and Heazell, {A. E P}",
year = "2013",
month = "5",
doi = "10.1016/j.placenta.2013.02.007",
language = "English",
volume = "34",
pages = "449--455",
journal = "Placenta",
issn = "0143-4004",
publisher = "Elsevier BV",
number = "5",

}

RIS

TY - JOUR

T1 - Syncytial nuclear aggregates in normal placenta show increased nuclear condensation, but apoptosis and cytoskeletal redistribution are uncommon

AU - Coleman, S. J.

AU - Gerza, L.

AU - Jones, C. J P

AU - Sibley, C. P.

AU - Aplin, J. D.

AU - Heazell, A. E P

PY - 2013/5

Y1 - 2013/5

N2 - Introduction: Syncytial nuclear aggregates (SNAs) are increased in pregnancy complications; however, little is known about their origin or function. This study aimed to characterise SNAs in more detail than has been reported previously. Methods: Immunohistochemistry and morphological examination at the light and ultrastructural level were used to determine the nature and structure of SNAs. Results: SNAs comprising bridges and syncytial knots had similar frequency with 974 per mm3 of villous tissue (IQR 717-1193) and 833 per mm3 (IQR 766-1190), respectively while there were approximately four times as many sectioning artefacts than knots and bridges combined. SNAs had increased proportions of condensed nuclei compared to the remaining syncytiotrophoblast (33.3% vs. 8.9%) and decreased proportions of euchromatic nuclei (0.0% vs. 16.2%), as assessed by examination of an electron micrograph archive. SNAs showed little evidence of apoptosis, with weak positivity for the apoptosis markers M30-neoepitope at 16.6% and TUNEL at 10.0%; strong staining was rarely seen for either marker. Immunofluorescence demonstrated rare association of actin (α, β or γ) with SNAs, whereas tubulin was in close proximity to SNAs and cytokeratin was seen within and surrounding SNAs. Discussion: M30-positive SNAs traced through serial sections were significantly more likely to be syncytial knots or sectioning artefacts than bridges. Nuclei within SNAs showed signs consistent with degeneration; however, this is unlikely to be an apoptotic process. There are few changes in configuration of cytoskeletal proteins around SNAs. Conclusions: These data suggest that the biogenesis and functional significance of SNAs still require resolution. ©2013 Elsevier Ltd. All rights reserved.

AB - Introduction: Syncytial nuclear aggregates (SNAs) are increased in pregnancy complications; however, little is known about their origin or function. This study aimed to characterise SNAs in more detail than has been reported previously. Methods: Immunohistochemistry and morphological examination at the light and ultrastructural level were used to determine the nature and structure of SNAs. Results: SNAs comprising bridges and syncytial knots had similar frequency with 974 per mm3 of villous tissue (IQR 717-1193) and 833 per mm3 (IQR 766-1190), respectively while there were approximately four times as many sectioning artefacts than knots and bridges combined. SNAs had increased proportions of condensed nuclei compared to the remaining syncytiotrophoblast (33.3% vs. 8.9%) and decreased proportions of euchromatic nuclei (0.0% vs. 16.2%), as assessed by examination of an electron micrograph archive. SNAs showed little evidence of apoptosis, with weak positivity for the apoptosis markers M30-neoepitope at 16.6% and TUNEL at 10.0%; strong staining was rarely seen for either marker. Immunofluorescence demonstrated rare association of actin (α, β or γ) with SNAs, whereas tubulin was in close proximity to SNAs and cytokeratin was seen within and surrounding SNAs. Discussion: M30-positive SNAs traced through serial sections were significantly more likely to be syncytial knots or sectioning artefacts than bridges. Nuclei within SNAs showed signs consistent with degeneration; however, this is unlikely to be an apoptotic process. There are few changes in configuration of cytoskeletal proteins around SNAs. Conclusions: These data suggest that the biogenesis and functional significance of SNAs still require resolution. ©2013 Elsevier Ltd. All rights reserved.

KW - Apoptosis

KW - Cytoskeletal proteins

KW - Syncytial bridges

KW - Syncytial knots

KW - Syncytiotrophoblast

U2 - 10.1016/j.placenta.2013.02.007

DO - 10.1016/j.placenta.2013.02.007

M3 - Article

VL - 34

SP - 449

EP - 455

JO - Placenta

JF - Placenta

SN - 0143-4004

IS - 5

ER -