siRNA liposome-gold nanorod vectors for multispectral optoacoustic tomography theranostics.

Research output: Contribution to journalArticle

  • External authors:
  • Adrian Taruttis
  • Neus Lozano
  • Antonio Nunes
  • Nicolas Beziere
  • Eva Herzog
  • Vasilis Ntziachristos

Abstract

Therapeutic applications of gene silencing using siRNA have seen increasing interest over the past decade. The optimization of the delivery and biodistribution of siRNA using liposome-gold nanorod (AuNRs) nanoscale carriers can greatly benefit from adept imaging methods that can visualize the time-resolved delivery performance of such vectors. In this work, we describe the effect of AuNR length incorporated with liposomes and show their complexation with siRNA as a novel gene delivery vehicle. We demonstrate the application of multispectral optoacoustic tomography (MSOT) to longitudinally visualize the localisation of siRNA carrying liposome-AuNR hybrids within tumors. Combination of in vivo MSOT with ex vivo fluorescence cryo-slice imaging offers further insight into the siRNA transport and activity obtained.

Bibliographical metadata

Original languageEnglish
JournalNanoscale
Volume6
Issue number22
DOIs
Publication statusPublished - 24 Oct 2014