Selective chemical intervention in the proteome of caenorhabditis elegansCitation formats

  • External authors:
  • Holger Husi
  • Fiona McAllister
  • Nicos Angelopoulos
  • Victoria J. Butler
  • Kevin R. Bailey
  • Kirk Malone
  • Logan MacKay
  • Paul Taylor
  • Antony P. Page
  • Malcolm Walkinshaw

Standard

Selective chemical intervention in the proteome of caenorhabditis elegans. / Husi, Holger; McAllister, Fiona; Angelopoulos, Nicos; Butler, Victoria J.; Bailey, Kevin R.; Malone, Kirk; MacKay, Logan; Taylor, Paul; Page, Antony P.; Turner, Nicholas J.; Barran, Perdita E.; Walkinshaw, Malcolm.

In: Journal of Proteome Research, Vol. 9, No. 11, 05.11.2010, p. 6060-6070.

Research output: Contribution to journalArticle

Harvard

Husi, H, McAllister, F, Angelopoulos, N, Butler, VJ, Bailey, KR, Malone, K, MacKay, L, Taylor, P, Page, AP, Turner, NJ, Barran, PE & Walkinshaw, M 2010, 'Selective chemical intervention in the proteome of caenorhabditis elegans' Journal of Proteome Research, vol. 9, no. 11, pp. 6060-6070. https://doi.org/10.1021/pr100427c

APA

Husi, H., McAllister, F., Angelopoulos, N., Butler, V. J., Bailey, K. R., Malone, K., ... Walkinshaw, M. (2010). Selective chemical intervention in the proteome of caenorhabditis elegans. Journal of Proteome Research, 9(11), 6060-6070. https://doi.org/10.1021/pr100427c

Vancouver

Husi H, McAllister F, Angelopoulos N, Butler VJ, Bailey KR, Malone K et al. Selective chemical intervention in the proteome of caenorhabditis elegans. Journal of Proteome Research. 2010 Nov 5;9(11):6060-6070. https://doi.org/10.1021/pr100427c

Author

Husi, Holger ; McAllister, Fiona ; Angelopoulos, Nicos ; Butler, Victoria J. ; Bailey, Kevin R. ; Malone, Kirk ; MacKay, Logan ; Taylor, Paul ; Page, Antony P. ; Turner, Nicholas J. ; Barran, Perdita E. ; Walkinshaw, Malcolm. / Selective chemical intervention in the proteome of caenorhabditis elegans. In: Journal of Proteome Research. 2010 ; Vol. 9, No. 11. pp. 6060-6070.

Bibtex

@article{d2f2c810da7942c5a4bb0ecd49e4cc90,
title = "Selective chemical intervention in the proteome of caenorhabditis elegans",
abstract = "We present the first study of protein regulation by ligands in Caenorhabditis elegans. The ligands were peptidyl-prolyl isomerase inhibitors of cyclophilins. Up-regulation is observed for several heat shock proteins and one ligand in particular caused a greater than 2-fold enhancement of cyclophilin CYN-5. Additionally, several metabolic enzymes display elevated levels. This approach, using label-free relative quantification, provides an extremely attractive way of measuring the effect of ligands on an entire proteome, with minimal sample pretreatment, which could be applicable to large-scale studies. In this initial study, which compares the effect of three ligands, 54 unique proteins have been identified that are up- (51) or down- (3) regulated in the presence of a given ligand. A total of 431 C. elegans proteins were identified. Our methodology provides an intriguing new direction for in vivo screening of the effects of novel and untested ligands at the whole organism level. {\circledC} 2010 American Chemical Society.",
keywords = "C. elegans, cyclophilin, label-free profiling, ligand intervention on intact proteome",
author = "Holger Husi and Fiona McAllister and Nicos Angelopoulos and Butler, {Victoria J.} and Bailey, {Kevin R.} and Kirk Malone and Logan MacKay and Paul Taylor and Page, {Antony P.} and Turner, {Nicholas J.} and Barran, {Perdita E.} and Malcolm Walkinshaw",
note = "675FA Times Cited:0 Cited References Count:52",
year = "2010",
month = "11",
day = "5",
doi = "10.1021/pr100427c",
language = "English",
volume = "9",
pages = "6060--6070",
journal = "Journal of Proteome Research",
issn = "1535-3893",
publisher = "American Chemical Society",
number = "11",

}

RIS

TY - JOUR

T1 - Selective chemical intervention in the proteome of caenorhabditis elegans

AU - Husi, Holger

AU - McAllister, Fiona

AU - Angelopoulos, Nicos

AU - Butler, Victoria J.

AU - Bailey, Kevin R.

AU - Malone, Kirk

AU - MacKay, Logan

AU - Taylor, Paul

AU - Page, Antony P.

AU - Turner, Nicholas J.

AU - Barran, Perdita E.

AU - Walkinshaw, Malcolm

N1 - 675FA Times Cited:0 Cited References Count:52

PY - 2010/11/5

Y1 - 2010/11/5

N2 - We present the first study of protein regulation by ligands in Caenorhabditis elegans. The ligands were peptidyl-prolyl isomerase inhibitors of cyclophilins. Up-regulation is observed for several heat shock proteins and one ligand in particular caused a greater than 2-fold enhancement of cyclophilin CYN-5. Additionally, several metabolic enzymes display elevated levels. This approach, using label-free relative quantification, provides an extremely attractive way of measuring the effect of ligands on an entire proteome, with minimal sample pretreatment, which could be applicable to large-scale studies. In this initial study, which compares the effect of three ligands, 54 unique proteins have been identified that are up- (51) or down- (3) regulated in the presence of a given ligand. A total of 431 C. elegans proteins were identified. Our methodology provides an intriguing new direction for in vivo screening of the effects of novel and untested ligands at the whole organism level. © 2010 American Chemical Society.

AB - We present the first study of protein regulation by ligands in Caenorhabditis elegans. The ligands were peptidyl-prolyl isomerase inhibitors of cyclophilins. Up-regulation is observed for several heat shock proteins and one ligand in particular caused a greater than 2-fold enhancement of cyclophilin CYN-5. Additionally, several metabolic enzymes display elevated levels. This approach, using label-free relative quantification, provides an extremely attractive way of measuring the effect of ligands on an entire proteome, with minimal sample pretreatment, which could be applicable to large-scale studies. In this initial study, which compares the effect of three ligands, 54 unique proteins have been identified that are up- (51) or down- (3) regulated in the presence of a given ligand. A total of 431 C. elegans proteins were identified. Our methodology provides an intriguing new direction for in vivo screening of the effects of novel and untested ligands at the whole organism level. © 2010 American Chemical Society.

KW - C. elegans

KW - cyclophilin

KW - label-free profiling

KW - ligand intervention on intact proteome

U2 - 10.1021/pr100427c

DO - 10.1021/pr100427c

M3 - Article

VL - 9

SP - 6060

EP - 6070

JO - Journal of Proteome Research

JF - Journal of Proteome Research

SN - 1535-3893

IS - 11

ER -