Selection of membrane protein targets for crystallization using PFO-PAGE electrophoresis

Research output: Contribution to journalArticlepeer-review

  • External authors:
  • R. M. Cleverley
  • M. Saleem
  • J. Kean

Abstract

A method to rapidly assess the oligomeric composition of multimeric proteins is notably absent from reported schemes for high throughput production and crystallization of membrane proteins. In this report we have investigated the suitability of PFO-PAGE electrophoresis for this purpose and present examples where it proves highly informative in selecting conditions favouring the functional oligomeric state of the target protein. Features such as the ability to analyze several samples in parallel, including crude membrane extracts, suggest it will be highly adaptable to high throughput analysis of membrane proteins. © 2008 Informa UK Ltd.

Bibliographical metadata

Original languageEnglish
Pages (from-to)625-630
Number of pages5
JournalMolecular Membrane Biology
Volume25
Issue number8
DOIs
Publication statusPublished - Dec 2008