Role of laccase as an enzymatic pretreatment method to improve lignocellulosic saccharificationCitation formats

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Role of laccase as an enzymatic pretreatment method to improve lignocellulosic saccharification. / Heap, Lucy; Green, Anthony; Brown, David; van Dongen, B E; Turner, Nicholas.

In: Catalysis Science and Technology, Vol. 4, No. 8, 2014, p. 2251-2259.

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Heap, Lucy ; Green, Anthony ; Brown, David ; van Dongen, B E ; Turner, Nicholas. / Role of laccase as an enzymatic pretreatment method to improve lignocellulosic saccharification. In: Catalysis Science and Technology. 2014 ; Vol. 4, No. 8. pp. 2251-2259.

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@article{ce4a666f061f49259550fb791cbc1fe4,
title = "Role of laccase as an enzymatic pretreatment method to improve lignocellulosic saccharification",
abstract = "The recalcitrant nature of lignocellulose, in particular due to the presence of lignin, is found to decrease the efficiency of cellulases during the saccharification of biomass. The efficient and cost effective removal of lignin is currently a critical biotechnological challenge in order to improve the enzymatic digestibility of cellulose for bioethanol production. In this study the role and reactivity of laccase from Trametes versicolor (TvL) was assessed with and without mediators for the improved saccharification of acid-pretreated wheat straw. Lignin model compound studies using veratryl alcohol and β-O-4 dimers revealed that 1-hydroxybenzotriazole (1-HBT) was the most effective mediator. Combination of TvL and TvL + 1-HBT treatments with an alkaline-peroxide extraction step increased the released glucose concentration following hydrolysis by up to 2.3 g L-1 compared to an untreated control. Pyrolysis-gas chromatography-mass spectrometry (py-GC-MS) with tetramethylammonium hydroxide (TMAH) thermochemolysis analysis of the extracted lignin revealed structural changes that are consistent with lignin degradation mechanisms typical of fungi. {\circledC} 2014 the Partner Organisations.",
author = "Lucy Heap and Anthony Green and David Brown and {van Dongen}, {B E} and Nicholas Turner",
year = "2014",
doi = "10.1039/c4cy00046c",
language = "English",
volume = "4",
pages = "2251--2259",
journal = "Catalysis Science and Technology",
issn = "2044-4753",
publisher = "Royal Society of Chemistry",
number = "8",

}

RIS

TY - JOUR

T1 - Role of laccase as an enzymatic pretreatment method to improve lignocellulosic saccharification

AU - Heap, Lucy

AU - Green, Anthony

AU - Brown, David

AU - van Dongen, B E

AU - Turner, Nicholas

PY - 2014

Y1 - 2014

N2 - The recalcitrant nature of lignocellulose, in particular due to the presence of lignin, is found to decrease the efficiency of cellulases during the saccharification of biomass. The efficient and cost effective removal of lignin is currently a critical biotechnological challenge in order to improve the enzymatic digestibility of cellulose for bioethanol production. In this study the role and reactivity of laccase from Trametes versicolor (TvL) was assessed with and without mediators for the improved saccharification of acid-pretreated wheat straw. Lignin model compound studies using veratryl alcohol and β-O-4 dimers revealed that 1-hydroxybenzotriazole (1-HBT) was the most effective mediator. Combination of TvL and TvL + 1-HBT treatments with an alkaline-peroxide extraction step increased the released glucose concentration following hydrolysis by up to 2.3 g L-1 compared to an untreated control. Pyrolysis-gas chromatography-mass spectrometry (py-GC-MS) with tetramethylammonium hydroxide (TMAH) thermochemolysis analysis of the extracted lignin revealed structural changes that are consistent with lignin degradation mechanisms typical of fungi. © 2014 the Partner Organisations.

AB - The recalcitrant nature of lignocellulose, in particular due to the presence of lignin, is found to decrease the efficiency of cellulases during the saccharification of biomass. The efficient and cost effective removal of lignin is currently a critical biotechnological challenge in order to improve the enzymatic digestibility of cellulose for bioethanol production. In this study the role and reactivity of laccase from Trametes versicolor (TvL) was assessed with and without mediators for the improved saccharification of acid-pretreated wheat straw. Lignin model compound studies using veratryl alcohol and β-O-4 dimers revealed that 1-hydroxybenzotriazole (1-HBT) was the most effective mediator. Combination of TvL and TvL + 1-HBT treatments with an alkaline-peroxide extraction step increased the released glucose concentration following hydrolysis by up to 2.3 g L-1 compared to an untreated control. Pyrolysis-gas chromatography-mass spectrometry (py-GC-MS) with tetramethylammonium hydroxide (TMAH) thermochemolysis analysis of the extracted lignin revealed structural changes that are consistent with lignin degradation mechanisms typical of fungi. © 2014 the Partner Organisations.

U2 - 10.1039/c4cy00046c

DO - 10.1039/c4cy00046c

M3 - Article

VL - 4

SP - 2251

EP - 2259

JO - Catalysis Science and Technology

JF - Catalysis Science and Technology

SN - 2044-4753

IS - 8

ER -