Rewiring Riboswitches to Create New Genetic Circuits in Bacteria

Research output: Contribution to journalArticle

  • Authors:
  • Christopher J. Robinson
  • D Medina-Stacey
  • M-C Wu
  • H A Vincent
  • J Micklefield

Abstract

Riboswitches are RNA elements that control the expression of genes through a variety of mechanisms in response to the specific binding of small-molecule ligands. Since their discovery, riboswitches have shown promise for the artificial control of transcription or translation of target genes, be it for industrial biotechnology, protein expression, metabolic engineering, antimicrobial target validation, or gene function discovery. However, natural riboswitches are often unsuitable for these purposes due to their regulation by small molecules which are already present within the cell. For this reason, research has focused on creating riboswitches that respond to alternative biologically inert ligands or to molecules which are of interest for biosensing. Here we present methods for the development of artificial riboswitches in Gram-negative and Gram-positive bacteria. These methods are based on reengineering natural aptamers to change their ligand specificity toward molecules which do not bind the original aptamer (ie, that are orthogonal to the original). The first approach involves targeted mutagenesis of native riboswitches to change their specificity toward rationally designed synthetic ligand analogs. The second approach involves the fusion of previously validated orthogonal aptamers with native expression platforms to create novel chimeric riboswitches for the microbial target. We establish the applicability of these methods both for the control of exogenous genes as well as for the control of native genes.

Bibliographical metadata

Original languageEnglish
Pages (from-to)319-48
Number of pages30
JournalMethods in Enzymology
Volume575
Early online date8 Apr 2016
DOIs
StatePublished - 2016