Resin bead micro-UV-visible absorption spectroscopy

Research output: Contribution to journalArticle

  • External authors:
  • Shin Wong Lu
  • Fabrice Birembaut
  • William S. Brocklesby
  • Jeremy G. Frey
  • Mark Bradley

Abstract

The construction and design of a microscope coupled with a miniature UV-vis spectrometer is described. This was applied to the study of dyes linked to solid supports and displayed good correlation in spectral shape and λmax values when compared to the dyes in solution, as well as showing a linear relationship between dye loading and UV-vis absorbance. The spectral profiles of these dyes at various pH's were measured and used to determine the pKa of the dyes on the beads, which were compared with the pKa values of the dyes in solution, thus enabling the dye-loaded beads to act as pH sensors. © 2005 American Chemical Society.

Bibliographical metadata

Original languageEnglish
Pages (from-to)2247-2251
Number of pages4
JournalAnalytical Chemistry
Volume77
Issue number7
DOIs
Publication statusPublished - 1 Apr 2005