Real-time screening of biocatalysts in live bacterial colonies

Research output: Contribution to journalArticle

  • External authors:
  • Cunyu Yan
  • Emrys A. Jones
  • Emmanuelle Claude
  • Shaneela Hussain

Abstract

Screening of bacterial colonies to identify new biocatalytic activities is a widely adopted tool in biotechnology, but is constrained by the requirements for colorimetric or tag-based detection methods. Herein we report a label-free screening platform using desorption electrospray ionization coupled with ion mobility mass spectrometry imaging (DESI-IM-MS). The screening method is demonstrated for both ammonia lyases and P450 monooxygenases expressed within live bacterial colonies and is shown to enable multiplexing of enzyme variants and substrate libraries simultaneously.

Bibliographical metadata

Original languageEnglish
Pages (from-to)1408-1411
Number of pages4
JournalJournal of the American Chemical Society
Volume139
Issue number4
Early online date13 Jan 2017
DOIs
StatePublished - 2017