Rapid determination of both the activity and enantioselectivity of ketoreductases

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Abstract

(Chemical Equation Presented) Fast and furious: A rapid and inexpensive assay for determining both the activity and enantioselectivity of ketoreductases (KREDs) has beendeveloped (see scheme; HRP = horseradish peroxidase, ABTS = 2,2′-azino-di(3-ethyl benzthiazoline-6-sulfonic acid). This assay, which employs an enantioselective alcohol oxidase as a reporter enzyme, was used to screen a panel of 17 KREDs in only 10 min using less than 0.5 mg substrate. © 2008 Wiley-VCH Verlag GmbH & Co. KGaA.

Bibliographical metadata

Original languageEnglish
Pages (from-to)2639-2641
Number of pages2
JournalAngewandte Chemie - International Edition
Volume47
Issue number14
DOIs
Publication statusPublished - 25 Mar 2008