Rapid and sensitive monitoring of biocatalytic reactions using ion mobility mass spectrometry

Research output: Contribution to journalArticle

  • External authors:
  • Cunyu Yan
  • Jason Schmidberger
  • Shaneela Hussain

Abstract

The combination of stable isotope labelling with direct infusion ion mobility mass spectrometry (IM-MS) enabled qualitative and quantitative monitoring of biocatalytic reactions with reduced analysis times, enhanced sensitivity and μL-level assay volumes. The new approach was demonstrated by applying to both lipase and monooxygenase enzymes, including multi-substrate screening.

Bibliographical metadata

Original languageEnglish
JournalAnalyst
Early online date16 Mar 2016
DOIs
StatePublished - 2016