Phosphodiesterase-5 inhibitors and omental and placental small artery function in normal pregnancy and pre-eclampsia

Research output: Contribution to journalArticle

  • External authors:
  • Mark Wareing
  • Maureen O'Hara
  • Louise C. Kenny
  • Michael J. Taggart
  • Laurence Skillern
  • Ian Machin
  • Philip N. Baker

Abstract

Objectives: In pre-eclampsia (PE), endothelium-dependent function of myometrial small arteries is markedly attenuated. The residual PE response is wholly NO mediated. We have previously demonstrated that PDE5 inhibition can improve endothelial function in myometrial small arteries from women with PE. We aimed to assess whether the effect of PDE5 inhibition in PE was myometrial artery specific. Study design: Small arteries were dissected from omental biopsies obtained at Caesarean section from normal pregnant women (NP, N = 20) and women with PE (N = 11). Chorionic plate small arteries were dissected from NP (N = 13) and PE (N = 11) placentae. Vasoconstriction (arginine vasopressin or thromboxane-mimetic U46619) and endothelial-dependent relaxation were assessed by wire and pressure myography. Constriction/relaxation curves were repeated post 1 h incubation with PDE5 inhibitors UK-343664 or sildenafil citrate (0, 10 or 100 nM). Results: Omental artery constriction was increased in PE. Omental vessel constriction was unaffected by PDE5 inhibition. Sildenafil citrate improved bradykinin-induced but not acetylcholine-induced relaxation of omental small arteries from NP women. PDE5 inhibition did not alter relaxation of omental arteries from women with PE. Placental small arteries were unaffected by PDE5 inhibition. Conclusion: Use of PDE5 inhibitors does not significantly alter endothelial-dependent relaxation in omental or placental small arteries from PE women. © 2004 Elsevier Ireland Ltd. All rights reserved.

Bibliographical metadata

Original languageEnglish
Pages (from-to)41-49
Number of pages8
JournalEuropean Journal of Obstetrics Gynecology and Reproductive Biology
Volume127
Issue number1
DOIs
Publication statusPublished - 1 Jul 2006