Personal exposure to house dust mite allergen in bed: Nasal air sampling and reservoir allergen levels

Research output: Contribution to journalArticle

  • Authors:
  • Robin Gore
  • R. B. Gore
  • E. A. Hadi
  • M. Craven
  • F. I. Smillie
  • And 4 others
  • External authors:
  • T. J. O'Meara
  • E. R. Tovey
  • A. Woodcock
  • A. Custovic

Abstract

Background: Assessment of personal exposure to dust mite allergen has relied on proxy measures. Only recently has a means to directly measure inhaled allergen particle number become available (the intra-nasal air sampler). Objective: To quantify inspired dust mite group 1 and group 2 allergen-bearing particles in bed in undisturbed conditions prior to sleep by nasal air sampling and to investigate the relationship between inhaled particles and reservoir allergen levels. Methods: Twelve volunteers wore nasal samplers in bed for 6 evenings, nose-breathing in undisturbed conditions. Allergen-bearing particles ('halos') were detected by immunostaining for Der p 1, Der p 2, or Der p 1 and Der p 2 together, and counted by light microscopy. Count data were square root transformed for analysis of variance. Mattress dust samples were assayed for Der p 1 and Der p 2 concentrations. Results: Square root detransformed mean particle counts per 30-min sample were: Der p 1, 4.22; Der p 2, 5.9; Der p 1 + Der p 2, 4.87; and for all samples, 5.01, with no difference between the groups. With replicate samples, halo number correlated significantly with mattress allergen concentrations (Der p 1 r=0.80, P

Bibliographical metadata

Original languageEnglish
Pages (from-to)856-859
Number of pages3
JournalClinical and Experimental Allergy
Volume32
Issue number6
DOIs
Publication statusPublished - 2002