Performance of LDBio Aspergillus WB and ICT Antibody Detection in Chronic Pulmonary Aspergillosis

Research output: Contribution to journalArticlepeer-review

  • External authors:
  • Anna Rozaliyani
  • Findra Setianingrum
  • Sresta Azahra
  • Asriyani Abdullah
  • Ayu Eka Fatril
  • Harmi Rosianawati
  • Erlina Burhan
  • Diah Handayani
  • Arief Riadi Arifin
  • Jamal Zaini
  • Mulyati Tugiran
  • robiatul adawiyah
  • Ridhawati Syam
  • Rona Kartika
  • Retno Wahyuningsih

Abstract

The detection of Aspergillus antibody has a key role in the diagnosis of chronic pulmonary as-pergillosis. Western blot (WB) and immunochromatography (ICT) lateral flow detection of Aspergillus antibody can be used as confirmatory and screening assays but their comparative performance in TB patients is not known. This study investigated the performance of these assays among 88 post-tuberculosis patients with suspected CPA. Sensitivity, specificity, receiver operating curve (ROC), area under-curve (AUC) and the agreement between two assays were evaluated. Both WB and ICT showed good sensitivity (80% and 85%, respectively) for detection of Aspergillus antibodies. Substantial agreement (0.716) between these assays was also obtained. The highest AUC result (0.804) was achieved with the combination of WB and ICT. The global intensity of WB correlated with the severity of symptoms in CPA group (p = 0.001). The combination of WB and ICT may increase specificity in CPA diagnosis.

Bibliographical metadata

Original languageEnglish
Article number311
JournalJournal of Fungi
Volume7
Issue number4
DOIs
Publication statusPublished - 18 Apr 2021

Related information