Orexin excites GABAergic neurons of the arcuate nucleus by activating the sodium-calcium exchangerCitation formats

  • Authors:
  • Denis Burdakov
  • Birgit Liss
  • Frances M. Ashcroft

Standard

Orexin excites GABAergic neurons of the arcuate nucleus by activating the sodium-calcium exchanger. / Burdakov, Denis; Liss, Birgit; Ashcroft, Frances M.

In: Journal of Neuroscience, Vol. 23, No. 12, 15.06.2003, p. 4951-4957.

Research output: Contribution to journalArticle

Harvard

Burdakov, D, Liss, B & Ashcroft, FM 2003, 'Orexin excites GABAergic neurons of the arcuate nucleus by activating the sodium-calcium exchanger', Journal of Neuroscience, vol. 23, no. 12, pp. 4951-4957.

APA

Burdakov, D., Liss, B., & Ashcroft, F. M. (2003). Orexin excites GABAergic neurons of the arcuate nucleus by activating the sodium-calcium exchanger. Journal of Neuroscience, 23(12), 4951-4957.

Vancouver

Burdakov D, Liss B, Ashcroft FM. Orexin excites GABAergic neurons of the arcuate nucleus by activating the sodium-calcium exchanger. Journal of Neuroscience. 2003 Jun 15;23(12):4951-4957.

Author

Burdakov, Denis ; Liss, Birgit ; Ashcroft, Frances M. / Orexin excites GABAergic neurons of the arcuate nucleus by activating the sodium-calcium exchanger. In: Journal of Neuroscience. 2003 ; Vol. 23, No. 12. pp. 4951-4957.

Bibtex

@article{980751dac1d44cbebc37fcb4bee382d6,
title = "Orexin excites GABAergic neurons of the arcuate nucleus by activating the sodium-calcium exchanger",
abstract = "The neuropeptides orexins/hypocretins are essential for normal wakefulness and energy balance, and disruption of their function causes narcolepsy and obesity. Although much is known of the role of orexins in sleep/wake behavior, it remains unclear how they stimulate feeding and metabolism. One of the main targets of orexinergic neurons is the arcuate nucleus (ARC) of the hypothalamus, which plays a key role in feeding and energy homeostasis. By combining patch-clamp an d RT-multiplex PCR analysis of individual neurons in mouse brain slices, we show that an electrophysiologically distinct subset of ARC neurons coexpress orexin receptors and glutamate decarboxylase-67 and are excited by orexin. Acting on postsynaptic orexin type 2 receptors, orexin activates a sodium-calcium exchange current, thereby depolarizing the cell and increasing its firing frequency. Because GABA is a potent stimulus for feeding, in both the ARC and its main projection site, these results suggest a mechanism for how orex in may control appetite.",
keywords = "Arcuate nucleus, Energy balance, Feeding, Hypocretin, Hypothalamus, Neuropeptides, Orexin, Sleep",
author = "Denis Burdakov and Birgit Liss and Ashcroft, {Frances M.}",
year = "2003",
month = jun
day = "15",
language = "English",
volume = "23",
pages = "4951--4957",
journal = "The Journal of Neuroscience",
issn = "0270-6474",
publisher = "Society for Neuroscience",
number = "12",

}

RIS

TY - JOUR

T1 - Orexin excites GABAergic neurons of the arcuate nucleus by activating the sodium-calcium exchanger

AU - Burdakov, Denis

AU - Liss, Birgit

AU - Ashcroft, Frances M.

PY - 2003/6/15

Y1 - 2003/6/15

N2 - The neuropeptides orexins/hypocretins are essential for normal wakefulness and energy balance, and disruption of their function causes narcolepsy and obesity. Although much is known of the role of orexins in sleep/wake behavior, it remains unclear how they stimulate feeding and metabolism. One of the main targets of orexinergic neurons is the arcuate nucleus (ARC) of the hypothalamus, which plays a key role in feeding and energy homeostasis. By combining patch-clamp an d RT-multiplex PCR analysis of individual neurons in mouse brain slices, we show that an electrophysiologically distinct subset of ARC neurons coexpress orexin receptors and glutamate decarboxylase-67 and are excited by orexin. Acting on postsynaptic orexin type 2 receptors, orexin activates a sodium-calcium exchange current, thereby depolarizing the cell and increasing its firing frequency. Because GABA is a potent stimulus for feeding, in both the ARC and its main projection site, these results suggest a mechanism for how orex in may control appetite.

AB - The neuropeptides orexins/hypocretins are essential for normal wakefulness and energy balance, and disruption of their function causes narcolepsy and obesity. Although much is known of the role of orexins in sleep/wake behavior, it remains unclear how they stimulate feeding and metabolism. One of the main targets of orexinergic neurons is the arcuate nucleus (ARC) of the hypothalamus, which plays a key role in feeding and energy homeostasis. By combining patch-clamp an d RT-multiplex PCR analysis of individual neurons in mouse brain slices, we show that an electrophysiologically distinct subset of ARC neurons coexpress orexin receptors and glutamate decarboxylase-67 and are excited by orexin. Acting on postsynaptic orexin type 2 receptors, orexin activates a sodium-calcium exchange current, thereby depolarizing the cell and increasing its firing frequency. Because GABA is a potent stimulus for feeding, in both the ARC and its main projection site, these results suggest a mechanism for how orex in may control appetite.

KW - Arcuate nucleus

KW - Energy balance

KW - Feeding

KW - Hypocretin

KW - Hypothalamus

KW - Neuropeptides

KW - Orexin

KW - Sleep

M3 - Article

VL - 23

SP - 4951

EP - 4957

JO - The Journal of Neuroscience

JF - The Journal of Neuroscience

SN - 0270-6474

IS - 12

ER -