‘One-pot’ sequential enzymatic modification of synthetic glycolipids in vesicle membranes

Research output: Contribution to journalArticle

  • External authors:
  • Faye Craven
  • Joana Silva
  • MD Segarra-Maset
  • Kun Huang
  • Peter Both

Abstract

β(1,4)-Galactosyltransferase (β4Gal-T1) and T. cruzi trans-sialidase (TcTS) have been used in a ‘one-pot’ cascade to provide vesicles (liposomes) with a trisaccharide coating. These soluble enzymes catalysed the transfer of galactose then sialic acid onto a synthetic N-acetylglucolipid embedded in the bilayers. Clustering of this substrate into microdomains increased the rate of sialylated lipid production, showing that an increase in β4Gal-T1 activity is carried through the enzymatic cascade. These coatings modulated cell recognition. Hepatocellular carcinoma cells took up vesicles modified by β4Gal-T1 alone more extensively than sialylated vesicles produced by ‘one-pot’ sequential enzymatic modification.

Bibliographical metadata

Original languageEnglish
JournalChemical Communications
Early online date4 Jan 2018
DOIs
StatePublished - 2018