Oncogenic Mutations Rewire Signaling Pathways by Switching Protein Recruitment to Phosphotyrosine Sites

Research output: Contribution to journalArticle

  • External authors:
  • Alicia Lundby
  • Giulia Franciosa
  • Kristina B. Emdal
  • Jan C. Refsgaard
  • Sebastian P. Gnosa
  • Dorte B. Bekker-jensen
  • Anna Secher
  • Svetlana R. Maurya
  • Indranil Paul
  • Blanca L. Mendez
  • Christian D. Kelstrup
  • Marie Kveiborg
  • Guillermo Montoya
  • Lars J. Jensen
  • Jesper V. Olsen

Abstract

Tyrosine phosphorylation regulates multi-layered signaling networks with broad implications in (patho)physiology, but high-throughput methods for functional annotation of phosphotyrosine sites are lacking. To decipher phosphotyrosine signaling directly in tissue samples, we developed a mass-spectrometry-based interaction proteomics approach. We measured the in vivo EGF-dependent signaling network in lung tissue quantifying >1,000 phosphotyrosine sites. To assign function to all EGF-regulated sites, we determined their recruited protein signaling complexes in lung tissue by interaction proteomics. We demonstrated how mutations near tyrosine residues introduce molecular switches that rewire cancer signaling networks, and we revealed oncogenic properties of such a lung cancer EGFR mutant. To demonstrate the scalability of the approach, we performed >1,000 phosphopeptide pulldowns and analyzed them by rapid mass spectrometric analysis, revealing tissue-specific differences in interactors. Our approach is a general strategy for functional annotation of phosphorylation sites in tissues, enabling in-depth mechanistic insights into oncogenic rewiring of signaling networks.

Bibliographical metadata

Original languageEnglish
Pages (from-to)543-560.e26
JournalCell
Volume179
Issue number2
Early online date3 Oct 2019
DOIs
Publication statusPublished - 3 Oct 2019