Nanoindentation of histological specimens using an extension of the Oliver and Pharr methodCitation formats

  • External authors:
  • Riaz Akhtar
  • Nick Bierwisch
  • Norbert Schwarzer

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Nanoindentation of histological specimens using an extension of the Oliver and Pharr method. / Akhtar, Riaz; Sherratt, Michael J.; Bierwisch, Nick; Derby, Brian; Mummery, Paul M.; Watson, Rachel E B; Schwarzer, Norbert.

In: Materials Research Society Symposium Proceedings, Vol. 1097, 2008, p. 33-38.

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Akhtar, Riaz ; Sherratt, Michael J. ; Bierwisch, Nick ; Derby, Brian ; Mummery, Paul M. ; Watson, Rachel E B ; Schwarzer, Norbert. / Nanoindentation of histological specimens using an extension of the Oliver and Pharr method. In: Materials Research Society Symposium Proceedings. 2008 ; Vol. 1097. pp. 33-38.

Bibtex

@article{5c907a545fdc42be83214322f7628e4c,
title = "Nanoindentation of histological specimens using an extension of the Oliver and Pharr method",
abstract = "The micro-mechanical properties of 5 μm thick histological sections of ferret aorta and vena cava were mapped as a function of distance from the outer adventitial layer using nanoindentation. In order to decouple the effect of the glass substrate on the elastic modulus of these thin sections, the nanoindentation data were analyzed using the extended Oliver and Pharr method which is readily accessible for coatings and layered materials with the software package, FilmDoctor{\textregistered}. In the aorta, the elastic modulus was found to decrease progressively from 35 MPa at the adventitia (outermost layer) to 8 MPa at the intima (innermost layer). This decrease in modulus was inversely correlated with elastic fibre density. In contrast, in the vena cava, the stiffest regions were found to be the adventitial (outer) and intimai (innermost) sections of the vessel cross-section. Both these regions were enriched in ECM components. The central region, thought to be largely cellular, had a relatively constant modulus of around 20 MPa. This study demonstrates that with this methodology it is possible to distinguish micro-mechanically between large arteries and veins, and therefore the same approach should allow age or disease related changes in the mechanical properties within a tissue to be quantified. {\textcopyright} 2008 Materials Research Society.",
author = "Riaz Akhtar and Sherratt, {Michael J.} and Nick Bierwisch and Brian Derby and Mummery, {Paul M.} and Watson, {Rachel E B} and Norbert Schwarzer",
year = "2008",
doi = "10.1557/PROC-1097-GG01-09",
language = "English",
volume = "1097",
pages = "33--38",
journal = "Materials Research Society Symposium Proceedings",
issn = "0272-9172",
publisher = "Materials Research Society",

}

RIS

TY - JOUR

T1 - Nanoindentation of histological specimens using an extension of the Oliver and Pharr method

AU - Akhtar, Riaz

AU - Sherratt, Michael J.

AU - Bierwisch, Nick

AU - Derby, Brian

AU - Mummery, Paul M.

AU - Watson, Rachel E B

AU - Schwarzer, Norbert

PY - 2008

Y1 - 2008

N2 - The micro-mechanical properties of 5 μm thick histological sections of ferret aorta and vena cava were mapped as a function of distance from the outer adventitial layer using nanoindentation. In order to decouple the effect of the glass substrate on the elastic modulus of these thin sections, the nanoindentation data were analyzed using the extended Oliver and Pharr method which is readily accessible for coatings and layered materials with the software package, FilmDoctor®. In the aorta, the elastic modulus was found to decrease progressively from 35 MPa at the adventitia (outermost layer) to 8 MPa at the intima (innermost layer). This decrease in modulus was inversely correlated with elastic fibre density. In contrast, in the vena cava, the stiffest regions were found to be the adventitial (outer) and intimai (innermost) sections of the vessel cross-section. Both these regions were enriched in ECM components. The central region, thought to be largely cellular, had a relatively constant modulus of around 20 MPa. This study demonstrates that with this methodology it is possible to distinguish micro-mechanically between large arteries and veins, and therefore the same approach should allow age or disease related changes in the mechanical properties within a tissue to be quantified. © 2008 Materials Research Society.

AB - The micro-mechanical properties of 5 μm thick histological sections of ferret aorta and vena cava were mapped as a function of distance from the outer adventitial layer using nanoindentation. In order to decouple the effect of the glass substrate on the elastic modulus of these thin sections, the nanoindentation data were analyzed using the extended Oliver and Pharr method which is readily accessible for coatings and layered materials with the software package, FilmDoctor®. In the aorta, the elastic modulus was found to decrease progressively from 35 MPa at the adventitia (outermost layer) to 8 MPa at the intima (innermost layer). This decrease in modulus was inversely correlated with elastic fibre density. In contrast, in the vena cava, the stiffest regions were found to be the adventitial (outer) and intimai (innermost) sections of the vessel cross-section. Both these regions were enriched in ECM components. The central region, thought to be largely cellular, had a relatively constant modulus of around 20 MPa. This study demonstrates that with this methodology it is possible to distinguish micro-mechanically between large arteries and veins, and therefore the same approach should allow age or disease related changes in the mechanical properties within a tissue to be quantified. © 2008 Materials Research Society.

U2 - 10.1557/PROC-1097-GG01-09

DO - 10.1557/PROC-1097-GG01-09

M3 - Article

VL - 1097

SP - 33

EP - 38

JO - Materials Research Society Symposium Proceedings

JF - Materials Research Society Symposium Proceedings

SN - 0272-9172

ER -