Multi-enzymes Production Studies in Single Tray Solid State Fermentation with Opened and Closed SystemCitation formats

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Multi-enzymes Production Studies in Single Tray Solid State Fermentation with Opened and Closed System. / Abdul Manan, Musaalbakri; Webb, Colin.

In: Journal of Life Sciences, Vol. 10, 2016, p. 342-356.

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Abdul Manan, Musaalbakri ; Webb, Colin. / Multi-enzymes Production Studies in Single Tray Solid State Fermentation with Opened and Closed System. In: Journal of Life Sciences. 2016 ; Vol. 10. pp. 342-356.

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@article{9f88eafccd2d4a779e91eacaa748cfc1,
title = "Multi-enzymes Production Studies in Single Tray Solid State Fermentation with Opened and Closed System",
abstract = "The robustness of A. awamori and A. oryzae as enzyme producers is exploited in fungal fermentation on agricultural solid waste. High-level production of extracellular glucoamylase, protease, cellulase and xylanase has been achieved. Three different types of {\textquoteleft}waste{\textquoteright} solids (wheat bran, soybean hulls and rapeseed meal) have been used in studies of solid state fermentation (SSF). The enzymes could be produced in significant levels by continuously supplying oxygen (O2) through the tray system known as “closed” and “opened” tray systems. A perforated tray system was developed in this study that permits direct access to O2. Testing the tray system with different perforated mesh aperture sizes in this study did not yield different results in growth performance of A. awamori and A. oryzae. A. awamori and A. oryzae can be very versatile in producing various enzymes with different substrates with different starch, protein, hemicellulose and cellulose contents. These studies indicate that A. awamori is more suitable for the efficient production of multiple enzymes in the closed system including xylanase and cellulase, while the production of glucoamylase and protease is superior in the opened system. A. oryzae is more suitable for the efficient production of protease and cellulase in the closed system, while the production of protease is more favourable the opened system. A. awamori efficiently consumed starch in wheat bran medium and produced very high glucoamylase activity, and after that, the fungus efficiently produced other enzymes to degrade other complex nutrients such as protein, hemicellulose and cellulose. Meanwhile, A. oryzae efficiently consumed protein in rapeseed meal and produced very high protease activity. The ability of both filamentous fungi, to convert biomass through SSF bioconversion will have a great impact on food and agro-industry in every aspect of life from food and medicine to fuel. ",
keywords = "Tray system , solid state fermentation , Aspergillus awamori , Aspergillus oryzae , glucoamylase , protease , xylanase , cellulase",
author = "{Abdul Manan}, Musaalbakri and Colin Webb",
year = "2016",
doi = "10.17265/1934-7391/2016.07.005",
language = "English",
volume = "10",
pages = "342--356",
journal = "Journal of Life Sciences",
issn = "1934-7391",
publisher = "David Publishing Co., Inc",

}

RIS

TY - JOUR

T1 - Multi-enzymes Production Studies in Single Tray Solid State Fermentation with Opened and Closed System

AU - Abdul Manan, Musaalbakri

AU - Webb, Colin

PY - 2016

Y1 - 2016

N2 - The robustness of A. awamori and A. oryzae as enzyme producers is exploited in fungal fermentation on agricultural solid waste. High-level production of extracellular glucoamylase, protease, cellulase and xylanase has been achieved. Three different types of ‘waste’ solids (wheat bran, soybean hulls and rapeseed meal) have been used in studies of solid state fermentation (SSF). The enzymes could be produced in significant levels by continuously supplying oxygen (O2) through the tray system known as “closed” and “opened” tray systems. A perforated tray system was developed in this study that permits direct access to O2. Testing the tray system with different perforated mesh aperture sizes in this study did not yield different results in growth performance of A. awamori and A. oryzae. A. awamori and A. oryzae can be very versatile in producing various enzymes with different substrates with different starch, protein, hemicellulose and cellulose contents. These studies indicate that A. awamori is more suitable for the efficient production of multiple enzymes in the closed system including xylanase and cellulase, while the production of glucoamylase and protease is superior in the opened system. A. oryzae is more suitable for the efficient production of protease and cellulase in the closed system, while the production of protease is more favourable the opened system. A. awamori efficiently consumed starch in wheat bran medium and produced very high glucoamylase activity, and after that, the fungus efficiently produced other enzymes to degrade other complex nutrients such as protein, hemicellulose and cellulose. Meanwhile, A. oryzae efficiently consumed protein in rapeseed meal and produced very high protease activity. The ability of both filamentous fungi, to convert biomass through SSF bioconversion will have a great impact on food and agro-industry in every aspect of life from food and medicine to fuel.

AB - The robustness of A. awamori and A. oryzae as enzyme producers is exploited in fungal fermentation on agricultural solid waste. High-level production of extracellular glucoamylase, protease, cellulase and xylanase has been achieved. Three different types of ‘waste’ solids (wheat bran, soybean hulls and rapeseed meal) have been used in studies of solid state fermentation (SSF). The enzymes could be produced in significant levels by continuously supplying oxygen (O2) through the tray system known as “closed” and “opened” tray systems. A perforated tray system was developed in this study that permits direct access to O2. Testing the tray system with different perforated mesh aperture sizes in this study did not yield different results in growth performance of A. awamori and A. oryzae. A. awamori and A. oryzae can be very versatile in producing various enzymes with different substrates with different starch, protein, hemicellulose and cellulose contents. These studies indicate that A. awamori is more suitable for the efficient production of multiple enzymes in the closed system including xylanase and cellulase, while the production of glucoamylase and protease is superior in the opened system. A. oryzae is more suitable for the efficient production of protease and cellulase in the closed system, while the production of protease is more favourable the opened system. A. awamori efficiently consumed starch in wheat bran medium and produced very high glucoamylase activity, and after that, the fungus efficiently produced other enzymes to degrade other complex nutrients such as protein, hemicellulose and cellulose. Meanwhile, A. oryzae efficiently consumed protein in rapeseed meal and produced very high protease activity. The ability of both filamentous fungi, to convert biomass through SSF bioconversion will have a great impact on food and agro-industry in every aspect of life from food and medicine to fuel.

KW - Tray system

KW - solid state fermentation

KW - Aspergillus awamori

KW - Aspergillus oryzae

KW - glucoamylase

KW - protease

KW - xylanase

KW - cellulase

U2 - 10.17265/1934-7391/2016.07.005

DO - 10.17265/1934-7391/2016.07.005

M3 - Article

VL - 10

SP - 342

EP - 356

JO - Journal of Life Sciences

JF - Journal of Life Sciences

SN - 1934-7391

ER -