Multi-enzymes Production Studies in Single Tray Solid State Fermentation with Opened and Closed System

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Abstract

The robustness of A. awamori and A. oryzae as enzyme producers is exploited in fungal fermentation on agricultural solid waste. High-level production of extracellular glucoamylase, protease, cellulase and xylanase has been achieved. Three different types of ‘waste’ solids (wheat bran, soybean hulls and rapeseed meal) have been used in studies of solid state fermentation (SSF). The enzymes could be produced in significant levels by continuously supplying oxygen (O2) through the tray system known as “closed” and “opened” tray systems. A perforated tray system was developed in this study that permits direct access to O2. Testing the tray system with different perforated mesh aperture sizes in this study did not yield different results in growth performance of A. awamori and A. oryzae. A. awamori and A. oryzae can be very versatile in producing various enzymes with different substrates with different starch, protein, hemicellulose and cellulose contents. These studies indicate that A. awamori is more suitable for the efficient production of multiple enzymes in the closed system including xylanase and cellulase, while the production of glucoamylase and protease is superior in the opened system. A. oryzae is more suitable for the efficient production of protease and cellulase in the closed system, while the production of protease is more favourable the opened system. A. awamori efficiently consumed starch in wheat bran medium and produced very high glucoamylase activity, and after that, the fungus efficiently produced other enzymes to degrade other complex nutrients such as protein, hemicellulose and cellulose. Meanwhile, A. oryzae efficiently consumed protein in rapeseed meal and produced very high protease activity. The ability of both filamentous fungi, to convert biomass through SSF bioconversion will have a great impact on food and agro-industry in every aspect of life from food and medicine to fuel.

Bibliographical metadata

Original languageEnglish
Pages (from-to)342-356
JournalJournal of Life Sciences
Volume10
DOIs
Publication statusPublished - 2016