Methodological considerations for large-scale breath analysis studies: lessons from the U-BIOPRED severe asthma project

Research output: Contribution to journalArticlepeer-review

  • Authors:
  • the U-BIOPRED study group


Methods for breath sampling and analysis require robust quality assessment to minimise the risk of false discoveries. Planning large-scale multi-site breath metabolite profiling studies also requires careful consideration of systematic and random variation as a result of sampling and analysis techniques. In this study we use breath sample data from the recent U-BIOPRED cohort to evaluate and discuss some important methodological considerations such as batch variation and correction, variation between sites, storage and transportation, as well as inter-instrument analytical differences. Based on this we provide a summary of recommended best practices for new large scale multi-site studies.

Bibliographical metadata

Original languageEnglish
JournalJournal of Breath Research
Issue number1
Early online date1 Oct 2018
Publication statusPublished - 30 Oct 2018