Membrane transporter engineering in industrial biotechnology and whole cell biocatalysis

Research output: Research - peer-reviewArticle


Because they mainly do not involve chemical changes, membrane transporters have been a Cinderella subject in the biotechnology of small molecule production, but this is a serious oversight. Influx transporters contribute significantly to the flux towards product, and efflux transporters ensure the accumulation of product in the much greater extracellular space of fermentors. Programmes for improving biotechnological processes might therefore give greater consideration to transporters than may have been commonplace. Strategies for identifying important transporters include expression profiling, genome-wide knockout studies, stress-based selection, and the use of inhibitors. In addition, modern methods of directed evolution and synthetic biology, especially those effecting changes in energy coupling, offer huge opportunities for increasing the flux towards extracellular product formation by transporter engineering.

Bibliographical metadata

Original languageEnglish
Pages (from-to)237-246
JournalTrends in Biotechnology
Issue number4
StatePublished - Mar 2015