Membrane tension orchestrates rear retraction in matrix directed cell migrationCitation formats

  • External authors:
  • Henry de Belly
  • Ignacio Busnelli
  • Thomas Waring
  • Roshna V. Nair
  • Vanesa Sokleva
  • Oana Dobre
  • Angus Cameron
  • Nils Gauthier
  • Chridtopher Lamaze
  • Aránzazu del Campo
  • Tobias Starborg
  • Tobias Zech
  • Jacky G. Goetz
  • Ewa K. Paluch

Standard

Membrane tension orchestrates rear retraction in matrix directed cell migration. / Hetmanski, Joseph; de Belly, Henry; Busnelli, Ignacio; Waring, Thomas ; Nair, Roshna V.; Sokleva, Vanesa; Dobre, Oana; Cameron, Angus; Gauthier, Nils; Lamaze, Chridtopher; Swift, Joe; del Campo, Aránzazu; Starborg, Tobias; Zech, Tobias; Goetz, Jacky G.; Paluch, Ewa K.; Schwartz, Jean-Marc; Caswell, Patrick.

In: Developmental cell, 2019.

Research output: Contribution to journalArticle

Harvard

Hetmanski, J, de Belly, H, Busnelli, I, Waring, T, Nair, RV, Sokleva, V, Dobre, O, Cameron, A, Gauthier, N, Lamaze, C, Swift, J, del Campo, A, Starborg, T, Zech, T, Goetz, JG, Paluch, EK, Schwartz, J-M & Caswell, P 2019, 'Membrane tension orchestrates rear retraction in matrix directed cell migration', Developmental cell. https://doi.org/10.1016/j.devcel.2019.09.006

APA

Vancouver

Hetmanski J, de Belly H, Busnelli I, Waring T, Nair RV, Sokleva V et al. Membrane tension orchestrates rear retraction in matrix directed cell migration. Developmental cell. 2019. https://doi.org/10.1016/j.devcel.2019.09.006

Author

Hetmanski, Joseph ; de Belly, Henry ; Busnelli, Ignacio ; Waring, Thomas ; Nair, Roshna V. ; Sokleva, Vanesa ; Dobre, Oana ; Cameron, Angus ; Gauthier, Nils ; Lamaze, Chridtopher ; Swift, Joe ; del Campo, Aránzazu ; Starborg, Tobias ; Zech, Tobias ; Goetz, Jacky G. ; Paluch, Ewa K. ; Schwartz, Jean-Marc ; Caswell, Patrick. / Membrane tension orchestrates rear retraction in matrix directed cell migration. In: Developmental cell. 2019.

Bibtex

@article{f71487c451ae49ae85f799cf9dcbc4a0,
title = "Membrane tension orchestrates rear retraction in matrix directed cell migration",
abstract = "In development, wound healing, and cancer metastasis, vertebrate cells move through 3D interstitial matrix, responding to chemical and physical guidance cues. Protrusion at the cell front has been extensively studied, but the retraction phase of the migration cycle is not well understood. Here, we show that fast-moving cells guided by matrix cues establish positive feedback control of rear retraction by sensing membrane tension. We reveal a mechanism of rear retraction in 3D matrix and durotaxis controlled by caveolae, which form in response to low membrane tension at the cell rear. Caveolae activate RhoA-ROCK1/PKN2 signaling via the RhoA guanidine nucleotide exchange factor (GEF) Ect2 to control local F-actin organization and contractility in this subcellular region and promote translocation of the cell rear. A positive feedback loop between cytoskeletal signaling and membrane tension leads to rapid retraction to complete the migration cycle in fast-moving cells, providing directional memory to drive persistent cell migration in complex matrices.",
author = "Joseph Hetmanski and {de Belly}, Henry and Ignacio Busnelli and Thomas Waring and Nair, {Roshna V.} and Vanesa Sokleva and Oana Dobre and Angus Cameron and Nils Gauthier and Chridtopher Lamaze and Joe Swift and {del Campo}, Ar{\'a}nzazu and Tobias Starborg and Tobias Zech and Goetz, {Jacky G.} and Paluch, {Ewa K.} and Jean-Marc Schwartz and Patrick Caswell",
year = "2019",
doi = "10.1016/j.devcel.2019.09.006",
language = "English",
journal = "Developmental cell",
issn = "1534-5807",
publisher = "Cell Press",

}

RIS

TY - JOUR

T1 - Membrane tension orchestrates rear retraction in matrix directed cell migration

AU - Hetmanski, Joseph

AU - de Belly, Henry

AU - Busnelli, Ignacio

AU - Waring, Thomas

AU - Nair, Roshna V.

AU - Sokleva, Vanesa

AU - Dobre, Oana

AU - Cameron, Angus

AU - Gauthier, Nils

AU - Lamaze, Chridtopher

AU - Swift, Joe

AU - del Campo, Aránzazu

AU - Starborg, Tobias

AU - Zech, Tobias

AU - Goetz, Jacky G.

AU - Paluch, Ewa K.

AU - Schwartz, Jean-Marc

AU - Caswell, Patrick

PY - 2019

Y1 - 2019

N2 - In development, wound healing, and cancer metastasis, vertebrate cells move through 3D interstitial matrix, responding to chemical and physical guidance cues. Protrusion at the cell front has been extensively studied, but the retraction phase of the migration cycle is not well understood. Here, we show that fast-moving cells guided by matrix cues establish positive feedback control of rear retraction by sensing membrane tension. We reveal a mechanism of rear retraction in 3D matrix and durotaxis controlled by caveolae, which form in response to low membrane tension at the cell rear. Caveolae activate RhoA-ROCK1/PKN2 signaling via the RhoA guanidine nucleotide exchange factor (GEF) Ect2 to control local F-actin organization and contractility in this subcellular region and promote translocation of the cell rear. A positive feedback loop between cytoskeletal signaling and membrane tension leads to rapid retraction to complete the migration cycle in fast-moving cells, providing directional memory to drive persistent cell migration in complex matrices.

AB - In development, wound healing, and cancer metastasis, vertebrate cells move through 3D interstitial matrix, responding to chemical and physical guidance cues. Protrusion at the cell front has been extensively studied, but the retraction phase of the migration cycle is not well understood. Here, we show that fast-moving cells guided by matrix cues establish positive feedback control of rear retraction by sensing membrane tension. We reveal a mechanism of rear retraction in 3D matrix and durotaxis controlled by caveolae, which form in response to low membrane tension at the cell rear. Caveolae activate RhoA-ROCK1/PKN2 signaling via the RhoA guanidine nucleotide exchange factor (GEF) Ect2 to control local F-actin organization and contractility in this subcellular region and promote translocation of the cell rear. A positive feedback loop between cytoskeletal signaling and membrane tension leads to rapid retraction to complete the migration cycle in fast-moving cells, providing directional memory to drive persistent cell migration in complex matrices.

U2 - 10.1016/j.devcel.2019.09.006

DO - 10.1016/j.devcel.2019.09.006

M3 - Article

JO - Developmental cell

JF - Developmental cell

SN - 1534-5807

ER -