Mapping the substrate scope of monoamine oxidase (MAO-N) as a synthetic tool for the enantioselective synthesis of chiral amines

Research output: Contribution to journalArticle

  • External authors:
  • Susanne Herter
  • Florian Medina
  • Simon Wagschal
  • Cyril Benhaïm
  • Friedemann Leipold

Abstract

A library of 132 racemic chiral amines (α-substituted methylbenzylamines, benzhydrylamines, 1,2,3,4-tetrahydronaphthylamines (THNs), indanylamines, allylic and homoallylic amines, propargyl amines) was screened against the most versatile monoamine oxidase (MAO-N) variants D5, D9 and D11. MAO-N D9 exhibited the highest activity for most substrates and was applied to the deracemisation of a comprehensive set of selected primary amines. In all cases, excellent enantioselectivity was achieved (e.e. >99%) with moderate to good yields (55-80%). Conditions for the deracemisation of primary amines using a MAO-N/borane system were further optimised using THN as a template addressing substrate load, nature of the enzyme preparation, buffer systems, borane sources, and organic co-solvents.

Bibliographical metadata

Original languageEnglish
JournalBioorganic and Medicinal Chemistry
Early online date13 Jul 2017
DOIs
Publication statusPublished - 2017