Liposome-Indocyanine Green Nanoprobes for Optical Labeling and Tracking of Human Mesenchymal Stem Cells Post-Transplantation In VivoCitation formats

  • External authors:
  • Mariarosa Mazza
  • Maria De Les Neus Lozano Valdes
  • Debora Braga Vieira
  • Maurizio Buggio

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Liposome-Indocyanine Green Nanoprobes for Optical Labeling and Tracking of Human Mesenchymal Stem Cells Post-Transplantation In Vivo. / Mazza, Mariarosa; Lozano Valdes, Maria De Les Neus; Braga Vieira, Debora; Buggio, Maurizio; Kielty, Cay; Kostarelos, Kostas.

In: Advanced Healthcare Materials, 2017.

Research output: Contribution to journalArticle

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Mazza, Mariarosa ; Lozano Valdes, Maria De Les Neus ; Braga Vieira, Debora ; Buggio, Maurizio ; Kielty, Cay ; Kostarelos, Kostas. / Liposome-Indocyanine Green Nanoprobes for Optical Labeling and Tracking of Human Mesenchymal Stem Cells Post-Transplantation In Vivo. In: Advanced Healthcare Materials. 2017.

Bibtex

@article{469785d87aa8472e8320a8d1c5d15282,
title = "Liposome-Indocyanine Green Nanoprobes for Optical Labeling and Tracking of Human Mesenchymal Stem Cells Post-Transplantation In Vivo",
abstract = "Direct labeling of human mesenchymal stem cells (hMSC) prior to transplantation provides a means to track cells after administration and it is a powerful tool for the assessment of new cell-based therapies. Biocompatible nanoprobes consisting of liposome-indocyanine green hybrid vesicles (liposome-ICG) are used to safely label hMSC. Labeled hMSC recapitulating a 3D cellular environment is transplanted as spheroids subcutaneously and intracranially in athymic nude mice. Cells emit a strong NIR signal used for tracking post-transplantation with the IVIS imaging system up to 2 weeks (subcutaneous) and 1 week (intracranial). The transplanted stem cells are imaged in situ after engraftment deep in the brain up to 1 week in living animals using optical imaging techniques and without the need to genetically modify the cells. This method is proposed for efficient, nontoxic direct cell labeling for the preclinical assessment of cell-based therapies and the design of clinical trials, and potentially for localization of the cell engraftment after transplantation into patients.",
keywords = "Cell tracking, Intracranial, Liposomes, Mesenchymal stromal cells, Spheroids, Subcutaneous",
author = "Mariarosa Mazza and {Lozano Valdes}, {Maria De Les Neus} and {Braga Vieira}, Debora and Maurizio Buggio and Cay Kielty and Kostas Kostarelos",
year = "2017",
doi = "10.1002/adhm.201700374",
language = "English",
journal = "Advanced Healthcare Materials",
issn = "2192-2640",
publisher = "John Wiley & Sons Ltd",

}

RIS

TY - JOUR

T1 - Liposome-Indocyanine Green Nanoprobes for Optical Labeling and Tracking of Human Mesenchymal Stem Cells Post-Transplantation In Vivo

AU - Mazza, Mariarosa

AU - Lozano Valdes, Maria De Les Neus

AU - Braga Vieira, Debora

AU - Buggio, Maurizio

AU - Kielty, Cay

AU - Kostarelos, Kostas

PY - 2017

Y1 - 2017

N2 - Direct labeling of human mesenchymal stem cells (hMSC) prior to transplantation provides a means to track cells after administration and it is a powerful tool for the assessment of new cell-based therapies. Biocompatible nanoprobes consisting of liposome-indocyanine green hybrid vesicles (liposome-ICG) are used to safely label hMSC. Labeled hMSC recapitulating a 3D cellular environment is transplanted as spheroids subcutaneously and intracranially in athymic nude mice. Cells emit a strong NIR signal used for tracking post-transplantation with the IVIS imaging system up to 2 weeks (subcutaneous) and 1 week (intracranial). The transplanted stem cells are imaged in situ after engraftment deep in the brain up to 1 week in living animals using optical imaging techniques and without the need to genetically modify the cells. This method is proposed for efficient, nontoxic direct cell labeling for the preclinical assessment of cell-based therapies and the design of clinical trials, and potentially for localization of the cell engraftment after transplantation into patients.

AB - Direct labeling of human mesenchymal stem cells (hMSC) prior to transplantation provides a means to track cells after administration and it is a powerful tool for the assessment of new cell-based therapies. Biocompatible nanoprobes consisting of liposome-indocyanine green hybrid vesicles (liposome-ICG) are used to safely label hMSC. Labeled hMSC recapitulating a 3D cellular environment is transplanted as spheroids subcutaneously and intracranially in athymic nude mice. Cells emit a strong NIR signal used for tracking post-transplantation with the IVIS imaging system up to 2 weeks (subcutaneous) and 1 week (intracranial). The transplanted stem cells are imaged in situ after engraftment deep in the brain up to 1 week in living animals using optical imaging techniques and without the need to genetically modify the cells. This method is proposed for efficient, nontoxic direct cell labeling for the preclinical assessment of cell-based therapies and the design of clinical trials, and potentially for localization of the cell engraftment after transplantation into patients.

KW - Cell tracking

KW - Intracranial

KW - Liposomes

KW - Mesenchymal stromal cells

KW - Spheroids

KW - Subcutaneous

UR - http://www.scopus.com/inward/record.url?scp=85026753875&partnerID=8YFLogxK

U2 - 10.1002/adhm.201700374

DO - 10.1002/adhm.201700374

M3 - Article

JO - Advanced Healthcare Materials

JF - Advanced Healthcare Materials

SN - 2192-2640

ER -