Lipase-catalysed acylation of starch and determination of the degree of substitution by methanolysis and GCCitation formats

  • Authors:
  • Apostolos Alissandratos
  • Nina Baudendistel
  • Sabine L. Flitsch
  • Bernhard Hauer
  • Peter J. Halling

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Lipase-catalysed acylation of starch and determination of the degree of substitution by methanolysis and GC. / Alissandratos, Apostolos; Baudendistel, Nina; Flitsch, Sabine L.; Hauer, Bernhard; Halling, Peter J.

In: BMC Biotechnology, Vol. 10, 82, 29.11.2010.

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Alissandratos, Apostolos ; Baudendistel, Nina ; Flitsch, Sabine L. ; Hauer, Bernhard ; Halling, Peter J. / Lipase-catalysed acylation of starch and determination of the degree of substitution by methanolysis and GC. In: BMC Biotechnology. 2010 ; Vol. 10.

Bibtex

@article{3182b6a95ba04a6b879fd987a0528f62,
title = "Lipase-catalysed acylation of starch and determination of the degree of substitution by methanolysis and GC",
abstract = "Background: Natural polysaccharides such as starch are becoming increasingly interesting as renewable starting materials for the synthesis of biodegradable polymers using chemical or enzymatic methods. Given the complexity of polysaccharides, the analysis of reaction products is challenging.Results: Esterification of starch with fatty acids has traditionally been monitored by saponification and back-titration, but in our experience this method is unreliable. Here we report a novel GC-based method for the fast and reliable quantitative determination of esterification. The method was used to monitor the enzymatic esterification of different starches with decanoic acid, using lipase from Thermomyces lanuginosus. The reaction showed a pronounced optimal water content of 1.25 mL per g starch, where a degree of substitution (DS) of 0.018 was obtained. Incomplete gelatinization probably accounts for lower conversion with less water.Conclusions: Lipase-catalysed esterification of starch is feasible in aqueous gel systems, but attention to analytical methods is important to obtain correct DS values. {\textcopyright} 2010 Alissandratos et al; licensee BioMed Central Ltd.",
author = "Apostolos Alissandratos and Nina Baudendistel and Flitsch, {Sabine L.} and Bernhard Hauer and Halling, {Peter J.}",
year = "2010",
month = nov,
day = "29",
doi = "10.1186/1472-6750-10-82",
language = "English",
volume = "10",
journal = "BMC Biotechnology",
issn = "1472-6750",
publisher = "Springer Nature",

}

RIS

TY - JOUR

T1 - Lipase-catalysed acylation of starch and determination of the degree of substitution by methanolysis and GC

AU - Alissandratos, Apostolos

AU - Baudendistel, Nina

AU - Flitsch, Sabine L.

AU - Hauer, Bernhard

AU - Halling, Peter J.

PY - 2010/11/29

Y1 - 2010/11/29

N2 - Background: Natural polysaccharides such as starch are becoming increasingly interesting as renewable starting materials for the synthesis of biodegradable polymers using chemical or enzymatic methods. Given the complexity of polysaccharides, the analysis of reaction products is challenging.Results: Esterification of starch with fatty acids has traditionally been monitored by saponification and back-titration, but in our experience this method is unreliable. Here we report a novel GC-based method for the fast and reliable quantitative determination of esterification. The method was used to monitor the enzymatic esterification of different starches with decanoic acid, using lipase from Thermomyces lanuginosus. The reaction showed a pronounced optimal water content of 1.25 mL per g starch, where a degree of substitution (DS) of 0.018 was obtained. Incomplete gelatinization probably accounts for lower conversion with less water.Conclusions: Lipase-catalysed esterification of starch is feasible in aqueous gel systems, but attention to analytical methods is important to obtain correct DS values. © 2010 Alissandratos et al; licensee BioMed Central Ltd.

AB - Background: Natural polysaccharides such as starch are becoming increasingly interesting as renewable starting materials for the synthesis of biodegradable polymers using chemical or enzymatic methods. Given the complexity of polysaccharides, the analysis of reaction products is challenging.Results: Esterification of starch with fatty acids has traditionally been monitored by saponification and back-titration, but in our experience this method is unreliable. Here we report a novel GC-based method for the fast and reliable quantitative determination of esterification. The method was used to monitor the enzymatic esterification of different starches with decanoic acid, using lipase from Thermomyces lanuginosus. The reaction showed a pronounced optimal water content of 1.25 mL per g starch, where a degree of substitution (DS) of 0.018 was obtained. Incomplete gelatinization probably accounts for lower conversion with less water.Conclusions: Lipase-catalysed esterification of starch is feasible in aqueous gel systems, but attention to analytical methods is important to obtain correct DS values. © 2010 Alissandratos et al; licensee BioMed Central Ltd.

U2 - 10.1186/1472-6750-10-82

DO - 10.1186/1472-6750-10-82

M3 - Article

VL - 10

JO - BMC Biotechnology

JF - BMC Biotechnology

SN - 1472-6750

M1 - 82

ER -