Lack of correlation between residual radiation-induced DNA damage, in keratinocytes assayed directly from skin, and late radiotherapy reactions in breast cancer patientsCitation formats

  • External authors:
  • Anne E. Kiltie
  • James B P Barber
  • Ric Swindell
  • Anderson J. Ryan
  • Jolyon H. Hendry
  • Brian Magee

Standard

Lack of correlation between residual radiation-induced DNA damage, in keratinocytes assayed directly from skin, and late radiotherapy reactions in breast cancer patients. / Kiltie, Anne E.; Barber, James B P; Swindell, Ric; Ryan, Anderson J.; West, Catharine M L; Hendry, Jolyon H.; Magee, Brian.

In: International Journal of Radiation Oncology Biology Physics, Vol. 43, No. 3, 01.02.1999, p. 481-487.

Research output: Contribution to journalArticlepeer-review

Harvard

Kiltie, AE, Barber, JBP, Swindell, R, Ryan, AJ, West, CML, Hendry, JH & Magee, B 1999, 'Lack of correlation between residual radiation-induced DNA damage, in keratinocytes assayed directly from skin, and late radiotherapy reactions in breast cancer patients', International Journal of Radiation Oncology Biology Physics, vol. 43, no. 3, pp. 481-487. https://doi.org/10.1016/S0360-3016(98)00392-7

APA

Kiltie, A. E., Barber, J. B. P., Swindell, R., Ryan, A. J., West, C. M. L., Hendry, J. H., & Magee, B. (1999). Lack of correlation between residual radiation-induced DNA damage, in keratinocytes assayed directly from skin, and late radiotherapy reactions in breast cancer patients. International Journal of Radiation Oncology Biology Physics, 43(3), 481-487. https://doi.org/10.1016/S0360-3016(98)00392-7

Vancouver

Kiltie AE, Barber JBP, Swindell R, Ryan AJ, West CML, Hendry JH et al. Lack of correlation between residual radiation-induced DNA damage, in keratinocytes assayed directly from skin, and late radiotherapy reactions in breast cancer patients. International Journal of Radiation Oncology Biology Physics. 1999 Feb 1;43(3):481-487. https://doi.org/10.1016/S0360-3016(98)00392-7

Author

Kiltie, Anne E. ; Barber, James B P ; Swindell, Ric ; Ryan, Anderson J. ; West, Catharine M L ; Hendry, Jolyon H. ; Magee, Brian. / Lack of correlation between residual radiation-induced DNA damage, in keratinocytes assayed directly from skin, and late radiotherapy reactions in breast cancer patients. In: International Journal of Radiation Oncology Biology Physics. 1999 ; Vol. 43, No. 3. pp. 481-487.

Bibtex

@article{fc0b9a6fcf2a4ff1bc2a70a8c901e358,
title = "Lack of correlation between residual radiation-induced DNA damage, in keratinocytes assayed directly from skin, and late radiotherapy reactions in breast cancer patients",
abstract = "Purpose: To study the relationship between the severity of late reactions to radiotherapy in breast cancer patients and the extent of residual radiation-induced DNA damage, using a rapid assay of keratinocytes obtained directly from skin biopsies. Methods and Materials: A review was made of 32 patients with breast cancer, treated uniformly by radiotherapy between 1983 following breast-conserving surgery. Their late radiotherapy reactions were scored (9-14 years post-radiotherapy) using a modified LENT SOMA scale, and a 5-mm buttock skin punch biopsy was obtained. Intact skin was irradiated at room temperature, and after allowing 24 h for repair, the tissue was disaggregated and the cells processed for pulsed field gel electrophoresis (PFGE). Residual DNA damage was expressed as the fraction of DNA released (FDR) following 150 Gy. Results: Studies using flow cytometry on disaggregated breast skin showed that over 90% of the cells were keratinocytes. The PFGE assay was robust with low background FDRs in unirradiated skin samples (mean 3.2%) and a wide range of FDRs following irradiation from 11.5% to 26.6%. No correlation was found between the FDR at 150 Gy (FDR 150) and any of the late reaction scores or retrospective acute reaction scores. There was, however, a borderline significant correlation for family history and FDR 150 (p = 0.059). Conclusion: Rapid measurement of residual DNA damage in irradiated differentiated keratinocytes, the predominant cell population in skin biopsies, showed no correlation with the severity of symptomatic early or documented late reactions in a retrospectively studied group of 32 breast cancer patients.",
keywords = "DNA damage, Keratinocytes, Normal tissues, Predictive assays, Radiosensitivity, Radiotherapy",
author = "Kiltie, {Anne E.} and Barber, {James B P} and Ric Swindell and Ryan, {Anderson J.} and West, {Catharine M L} and Hendry, {Jolyon H.} and Brian Magee",
year = "1999",
month = feb,
day = "1",
doi = "10.1016/S0360-3016(98)00392-7",
language = "English",
volume = "43",
pages = "481--487",
journal = "International Journal of Radiation: Oncology - Biology - Physics",
issn = "0360-3016",
publisher = "Elsevier BV",
number = "3",

}

RIS

TY - JOUR

T1 - Lack of correlation between residual radiation-induced DNA damage, in keratinocytes assayed directly from skin, and late radiotherapy reactions in breast cancer patients

AU - Kiltie, Anne E.

AU - Barber, James B P

AU - Swindell, Ric

AU - Ryan, Anderson J.

AU - West, Catharine M L

AU - Hendry, Jolyon H.

AU - Magee, Brian

PY - 1999/2/1

Y1 - 1999/2/1

N2 - Purpose: To study the relationship between the severity of late reactions to radiotherapy in breast cancer patients and the extent of residual radiation-induced DNA damage, using a rapid assay of keratinocytes obtained directly from skin biopsies. Methods and Materials: A review was made of 32 patients with breast cancer, treated uniformly by radiotherapy between 1983 following breast-conserving surgery. Their late radiotherapy reactions were scored (9-14 years post-radiotherapy) using a modified LENT SOMA scale, and a 5-mm buttock skin punch biopsy was obtained. Intact skin was irradiated at room temperature, and after allowing 24 h for repair, the tissue was disaggregated and the cells processed for pulsed field gel electrophoresis (PFGE). Residual DNA damage was expressed as the fraction of DNA released (FDR) following 150 Gy. Results: Studies using flow cytometry on disaggregated breast skin showed that over 90% of the cells were keratinocytes. The PFGE assay was robust with low background FDRs in unirradiated skin samples (mean 3.2%) and a wide range of FDRs following irradiation from 11.5% to 26.6%. No correlation was found between the FDR at 150 Gy (FDR 150) and any of the late reaction scores or retrospective acute reaction scores. There was, however, a borderline significant correlation for family history and FDR 150 (p = 0.059). Conclusion: Rapid measurement of residual DNA damage in irradiated differentiated keratinocytes, the predominant cell population in skin biopsies, showed no correlation with the severity of symptomatic early or documented late reactions in a retrospectively studied group of 32 breast cancer patients.

AB - Purpose: To study the relationship between the severity of late reactions to radiotherapy in breast cancer patients and the extent of residual radiation-induced DNA damage, using a rapid assay of keratinocytes obtained directly from skin biopsies. Methods and Materials: A review was made of 32 patients with breast cancer, treated uniformly by radiotherapy between 1983 following breast-conserving surgery. Their late radiotherapy reactions were scored (9-14 years post-radiotherapy) using a modified LENT SOMA scale, and a 5-mm buttock skin punch biopsy was obtained. Intact skin was irradiated at room temperature, and after allowing 24 h for repair, the tissue was disaggregated and the cells processed for pulsed field gel electrophoresis (PFGE). Residual DNA damage was expressed as the fraction of DNA released (FDR) following 150 Gy. Results: Studies using flow cytometry on disaggregated breast skin showed that over 90% of the cells were keratinocytes. The PFGE assay was robust with low background FDRs in unirradiated skin samples (mean 3.2%) and a wide range of FDRs following irradiation from 11.5% to 26.6%. No correlation was found between the FDR at 150 Gy (FDR 150) and any of the late reaction scores or retrospective acute reaction scores. There was, however, a borderline significant correlation for family history and FDR 150 (p = 0.059). Conclusion: Rapid measurement of residual DNA damage in irradiated differentiated keratinocytes, the predominant cell population in skin biopsies, showed no correlation with the severity of symptomatic early or documented late reactions in a retrospectively studied group of 32 breast cancer patients.

KW - DNA damage

KW - Keratinocytes

KW - Normal tissues

KW - Predictive assays

KW - Radiosensitivity

KW - Radiotherapy

U2 - 10.1016/S0360-3016(98)00392-7

DO - 10.1016/S0360-3016(98)00392-7

M3 - Article

VL - 43

SP - 481

EP - 487

JO - International Journal of Radiation: Oncology - Biology - Physics

JF - International Journal of Radiation: Oncology - Biology - Physics

SN - 0360-3016

IS - 3

ER -